| 川芎嗪联合创伤性脑组织匀浆液诱导骨髓间充质干细胞向神经样细胞分化 |
| |
| 引用本文： | 梁峰,马洁华,杨丽娜. 川芎嗪联合创伤性脑组织匀浆液诱导骨髓间充质干细胞向神经样细胞分化[J]. 中国组织工程研究与临床康复, 2011, 15(23). DOI: 10.3969/j.issn.1673-8225.2011.23.027 |
| |
| 作者姓名： | 梁峰 马洁华 杨丽娜 |
| |
| 作者单位： | 河北北方学院解剖调研室,河北省张家口市,075000 |
| |
| 基金项目： | 河北省教育厅资助课题(2007304).课题名称:胚胎神经干细胞修复脑创伤的实验研究 |
| |
| 摘 要： | 背景:川芎嗪和创伤性脑组织匀浆液均可诱导骨髓间充质干细胞向神经样细胞分化.目的:探讨川芎嗪与创伤性脑组织匀浆液诱导骨髓间充质干细胞向神经样细胞分化的联合效应.方法:分离培养Wistar大鼠骨髓间充质干细胞后分为4组,加入不同的诱导培养基分别干预:空白对照组、川芎嗪诱导组、创伤性脑匀浆液诱导组和川芎嗪联合创伤性脑匀浆液诱导组.诱导后采用倒差显微镜观察细胞形态变化,并统计不同时段四组细胞分化率.分别取部分细胞进行神经元特异性烯醇化酶染色,胶质纤维酸性蛋白免疫细胞化学与免疫荧光细胞化学双标检测.结果与结论:川芎嗪及受损大鼠脑匀浆液上清液可诱导大鼠骨髓间充质干细胞向神经元样细胞分化,随诱导时间的延长,诱导分化率越高,具有较重要的应用价值,而神经元特异性烯醇化酶与胶质纤维酸性蛋白在其分化信号中起重要作用.
|
| 关 键 词： | 川芎嗪 骨髓间充质干细胞 神经样细胞 创伤性脑组织匀浆液 胶质纤维酸性蛋白 |
Ligustrazine and traumatic brain tissue homogenate for inducing bone marrow mesenchymal stem cells differentiate into neuron-like cells |
| |
| Liang Feng,Ma Jie-hua,Yang Li-na. Ligustrazine and traumatic brain tissue homogenate for inducing bone marrow mesenchymal stem cells differentiate into neuron-like cells[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(23). DOI: 10.3969/j.issn.1673-8225.2011.23.027 |
| |
| Authors: | Liang Feng Ma Jie-hua Yang Li-na |
| |
| Affiliation: | Liang Feng,Ma Jie-hua,Yang Li-na Department of Anatomy,Hebei North University,Zhangjiakou 075000,Hebei Province,China |
| |
| Abstract: | BACKGROUND:Both ligustrazine and traumatic brain tissue homogenate could induce bone marrow mesenchymal stem cells(BMSCs) differentiate to neuron-like cells.OBJECTIVE:To investigate the combined effects of ligustrazine and traumatic brain tissue homogenate for inducing BMSCs differentiate into neuron-like cells.METHODS:BMSCs isolated from Wistar rats were randomly divided into four groups:blank control,ligustrazine induction,traumatic brain homogenate induction,and combined induction.The morphology of the c... |
| |
| Keywords: | |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
