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| 枸杞多糖对精原干细胞体外增殖的影响 | |
| 引用本文： | 马良宏,邱志军,闫圣男,冯立新,王燕蓉,李培军,陈福宝. 枸杞多糖对精原干细胞体外增殖的影响[J]. 中国组织工程研究与临床康复, 2011, 15(23): 4277-4281. DOI: 10.3969/j.issn.1673-8225.2011.23.022 |
| 作者姓名： | 马良宏 邱志军 闫圣男 冯立新 王燕蓉 李培军 陈福宝 |
| 作者单位： | 1. 宁夏医科大学附属医院泌尿外科,宁夏回族自治区银川市,750004 2. 宁夏医科大学,宁夏回族自治区银川市,750004 3. 上海交通大学医学院生殖干细胞实验室,上海市,200025 4. 宁夏医科大学生育力保持教育部重点实验室,宁夏回族自治区银川市,750004 |
| 基金项目： | 国家自然科学基金资助项目，宁夏自然科学基金资助项目 |
| 摘 要： | 背景:精原干细胞移植对不育具有潜在的临床应用价值,体外建立精原干细胞的培养系统获得数量较多的精原干细胞,仍是目前研究中亟待解决的问题。目的:观察枸杞多糖对精原干细胞体外增殖的影响。方法:采用两步酶消化法获取出生4～6d雄性C57BL/6小鼠睾丸Sertoli细胞与精原干细胞,将精原干细胞接种在Sertoli细胞饲养层上,再加入枸杞多糖或联合细胞因子添加到细胞培养液中。1周后以流式细胞仪检测细胞周期及细胞活性率,并检测各组精原干细胞GFRa-1、Thy-1、c-kit的阳性率。结果与结论:单独加入枸杞多糖后精原干细胞数量明显增加,增殖明显,联合加入胶质细胞源性神经营养因子与白血病抑制因子精原干细胞增殖更加明显(P<0.05)。并发现体外培养1周后的精原干细胞仍保持其睾丸组织内的精原干细胞特征,大多仍维持在未分化状态。表明在枸杞多糖或枸杞多糖联合胶质细胞源性神经营养因子及白血病抑制因子作用下,可促进精原干细胞体外增殖。 |
| 关 键 词： | 精原干细胞 枸杞多糖 体外 培养 增殖 |
Influence of lycium barbarum polysaccharides on proliferation of spermatogonial stem cells in vitro |
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| Ma Liang-hong,Qiu Zhi-jun,Yan Sheng-nan,Feng Li-xin,Wang Yan-rong,Li Pei-jun,Chen Fu-bao. Influence of lycium barbarum polysaccharides on proliferation of spermatogonial stem cells in vitro[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(23): 4277-4281. DOI: 10.3969/j.issn.1673-8225.2011.23.022 | |
| Authors: | Ma Liang-hong Qiu Zhi-jun Yan Sheng-nan Feng Li-xin Wang Yan-rong Li Pei-jun Chen Fu-bao |
| Affiliation: | Ma Liang-hong1,Qiu Zhi-jun2,Yan Sheng-nan2,Feng Li-xin3,Wang Yan-rong4,Li Pei-jun1,Chen Fu-bao1 1Department of Urology,Affiliated Hospital of Ningxia Medical University,Yinchuan 750004,Ningxia Hui Autonomous Region,China,2Ningxia Medical University,3Laboratory for Germline Stem Cell Research,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,4Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education in Ningxia Me... |
| Abstract: | BACKGROUND:It has potential clinical value to use spermatogonial stem cell transplantation to cure infertility. In order to obtain a large number of spermatogonial stem cells for transplantation,culture system in vitro need to be established,which is still an urgent problem at present. OBJECTIVE:To investigate the effect of lycium barbarum polysaccharides(LBP) on proliferation of spermatogonial stem cells in vitro. METHODS:Sertoli cells and spermatogonial stem cells were separated from testis of 4-6 days po... |
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