脂筏特异性AIE荧光探针的设计、合成及其应用

脂筏(lipid raft)是由饱和磷脂、鞘磷脂以及胆固醇组成的位于细胞膜的液态有序微区,与细胞的许多生理/病理过程密切相关。基于脂筏区与非脂筏区脂质组成与分布的差异,本研究设计并合成了一种具有聚集诱导发光(aggregationinduced emission,AIE)特性的脂筏探针胆固醇-三聚乙二醇-四苯乙烯(TCHS-TPE)用于细胞膜上脂筏区的便捷特异性成像。本研究首先成功合成了TCHS-TPE,同时测定了TCHS-TPE的光物理性质以评价其AIE特性,最后采用激光共聚焦显微镜研究了TCHS-TPE对B16F10黑色素瘤细胞膜上脂筏区的特异性成像。与现有脂筏探针霍乱毒素B(CTxB)相比,TCHS-TPE脂筏探针具有操作简单、特异性高等优势。该荧光探针的成功合成将为研究脂筏区相关生理、病理过程提供有利工具,并为其他脂筏区成像探针的设计奠定了理论基础。

Design, synthesis and application of AIE fluorescent probe for lipid raft

Lipid rafts composed of saturated phospholipids,sphingomyelin,and cholesterol are usually defined as liquid ordered microdomains located in the cell membrane. Lipid rafts are involved in many physiological and pathological processes of cells. Based on the difference in composition and distribution between lipid raft and non-raft domains,a lipid raft probe with aggregation-induced emission (AIE),cholesterol-triethylene glycol-tetraphenylethylene (TCHS-TPE),was designed and synthesized for convenient and specific imaging of lipid raft domains on cell membranes in this study. In this paper,TCHS-TPE was successfully synthesized,and the photophysical properties of TCHS-TPE were measured to evaluate its AIE characteristics. And finally the specific imaging of TCHS-TPE on the lipid raft region of B16F10 melanoma cell membrane was studied using confocal laser scanning microscopy. Compared with the existing lipid raft probe cholera toxin B (CTxB),the TCHS-TPE lipid raft probe has the advantages of simple operation and high specificity. The successful synthesis of the fluorescent probe will provide a useful tool for studying the physiological and pathological processes related to lipid raft domains,and offer a theoretical basis for the design of imaging probes for other lipid raft domains.