针刺及磁提针对骨骼肌静息电位恢复的作用

背景骨骼肌长时间收缩会导致静息单位的下降,影响其收缩,导致疲劳发生.针刺以及无损伤的磁提针对静息单位的恢复有无作用?目的采用针刺以及无损伤的磁提针作为恢复手段,观察和比较其对骨骼肌静息电位恢复的影响.设计对照实验.单位山西师范大学体育学院,山东省滨州学院.材料实验蟾蜍30只(由北京体育大学实验动物中心提供),随机分成针刺组、磁提针组、磁提针加按压组,每组10只.左右半腱肌进行对照.方法对离体蟾蜍半腱肌(实验肌和对照肌)进行40 min电刺激后,采用细胞内玻璃微电极技术测量骨骼肌细胞的静息电位.并且在电刺激后进行毫针针刺、磁提针和磁提针加按压等处理.主要观察指标各组运动前、电刺激后以及处理后的静息电位.结果①电刺激后骨骼肌细胞膜静息电位较电刺激前下降[实验肌(59.48±7.37),(84.54±5.72)mV,对照肌(59.43±7.14),(83.62±5.86)mV].②针刺、磁提针、磁提针加按压使骨骼肌细胞电刺激后下降的静息电位明显恢复(P＜0.01).⑧磁提针组静息电位恢复百分比明显小于针刺组和磁提针加按压组[(42.50±12.41)%,(53.71±10.93)%,(49.10±8.39)%,P＜0.01].结论斜刺、磁提针、磁提针加按压对长时间电刺激后骨骼肌细胞膜静息电位的恢复有促进作用.单纯磁提针对长时间电刺激后骨骼肌细胞膜静息电位恢复的促进作用小于针刺和磁提针加按压作用.

Effect of acupuncture and magnetic lifting needle on the recovery of resting potential of skeletal muscle

BACKGROUND:The long-term contraction of skeletal muscle can lead to the decrease of resting potential, affect its contraction, and result in the occurrence of fatigue. Whether acupuncture and injury-free magnetic lifting needle are effective on the recovery of resting potential? OBJECTIVE: To observe and compare the influence on the recovery of resting potential of skeletal muscle by taking acupuncture and magnetic needle as the recovery means.DESIGN: A controlled study.SETTINGS: College of Physical Education, Shanxi Normal University;Binzhou College of Shandong Province.MATERIALS: Thirty experimental toads, which were provided by Beijing University of Physical Education, were randomly divided into acupuncture group (n=10), magnetic needle group (n=10), magnetic needle+pressing group (n=10). Left and right semitendinosus muscles were taken as controls.METHODS: The in vitro toad's semitendinosus muscles (experimental side and control side) were treated with electrostimulation for 40 minutes,and then the resting potential of skeletal muscular cells were measured with the intracellular glass microelectrode technique. After the electrostimulation, filiform needle acupuncture, magnetic needle, magnetic needle plus pressing were also given.MAIN OUTCOME MEASURES: The resting potentials were observed before exercise, after electrostimulation and other treatments in the groups.RESULTS: ① The resting potentials of skeletal muscular cells after electrostimulation were decreased as compared with those before electrostimulation [experimental side: (59.48±7.37), (84.54±5.72) mV; control side:(59.43±7.14), (83.62±5.86) mV]. ② After the treatments of acupuncture,magnetic lifting needle, magnetic lifting needle plus pressing, the decreased resting potentials after electrostimulation were obviously recovered (P ＜ 0.01). ③ The recovered percentage of resting potential in the magnetic needle group was obviously smaller than those in the acupuncture group and magnetic needle+pressing group [(42.50±12.41)%, (53.71±10.93)%,(49.10±8.39)%, P ＜ 0. 01].CONCLUSION: Acupuncture, magnetic lifting needle, magnetic lifting needle plus pressing plays a role in promoting the recovery of resting potential of skeletal muscular cells after long-term electrostimulation, and the effect of simple magnetic needle is smaller than that of acupuncture and magnetic needle plus pressing.