DNA: DNA hybridization method for the diagnosis of hepatitis B infection |
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| Authors: | S V Feinman B Berris A Guha R Sooknanan D W Bradley W W Bond J E Maynard |
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| Affiliation: | 1. The Liver Study Unit, Mount Sinai Hospital, Department of Medicine. University of Toronto, Toronto, Ontario, Canada M5G 1X5;2. Department of Biology Erindale Campus, University of Toronto, Toronto, Ontario, Canada L5L 1C6;3. The Hepatitis Laboratories, Center for Disease Control, Phoenix, AZ 85014, U.S.A. |
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| Abstract: | Hepatitis B viral (HBV) DNA was detected in a hepatoma cell line which produces hepatitis B surface antigen (HBsAg) and in patients with acute hepatitis B. The serum of one patient with acute hepatitis B was found to be infectious when injected i.v. into a chimpanzee up to a dilution of 10(-8). Hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) were detectable in the same serum sample by radioimmunoassay up to a dilution of 10(-5) and of 10(-3), respectively. Using DNA: DNA hybridization on nitrocellulose membranes, HBV DNA sequences were detectable up to 10(-8) dilution corresponding to the infectivity level. Based on this finding, it appears that DNA: DNA hybridization is the most sensitive method for detecting hepatitis B virus (HBV) infection. In situations with low virus levels it may be the only indicator of the presence of infectious hepatitis B virus. The use of a tritium-labelled probe makes the method economical and adaptable to hospital laboratories. |
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| Keywords: | hepatitis B virus DNA DNA hybridization |
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