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Determination of the DDE and PCB contents of peregrine Falcon eggs: A comparison of whole egg measurements and estimates derived from eggshell membranes
Authors:David B. Peakal'  Thomas S. Lew  Alan M. Springer  Wayman Walker IP  Robert W. Risebrough  J. Geoffrey Monk  Walter M. Jarman  Brian J. Walton  Lincoln M. Reynolds  Richard W. Fyfe  Lloyd F. Kiff
Affiliation:(1) Department of the Environment, National Wildlife Research Centre, Canadian Wildlife Service, K1A 0E7 Ottawa, Ontario, Canada;(2) California Department of Fish and Game, Water Pollution Control Laboratory, 2005 Nimbus Road, 95670 Rancho Cordova, California;(3) Bodega Marine Laboratory, University of California, P.O. Box 247, 94923 Bodega Bay, California;(4) The Bodega Bay Institute, 2711 Piedmont Avenue, 94705 Berkeley, California;(5) Santa Cruz Predatory Bird Research Group, University of California, Clark Kerr Hall, 95064 Santa Cruz, California;(6) Ontario Research Foundation, Sheridan Park, L5K 1B3 Mississauga, Ontario, Canada;(7) Canadian Wildlife Service, #1000, 9942-108th Street, T5K 2J5 Edmonton, Alberta, Canada;(8) Western Foundation of Vertebrate Zoology, 1100 Glendon Avenue, 90024 Los Angeles, California
Abstract:A method using eggshell membranes to determine egg content levels of DDE and polychlorobiphenyls (PCBs), two of the most ubiquitous environmental contaminants, has been validated. A comparison was made between the residue levels determined from the egg contents of 40 Peregrine Falcon (Falco peregrinus) eggs and from hexanesoluble extracts of the dried eggshell membranes. The results confirm the use of museum eggshells for estimations of DDE and PCB levels of the original egg contents. This method should be valid for other lipophilic compounds. The data generated are of use not only to determine the records of past contamination, but may be expected to provide useful information on patterns of contamination encountered in different geographical areas. The membrane extraction technique allows pollutant levels to be measured without removing viable material from endangered species.
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