日本血吸虫抗原负载树突状细胞的表型研究

目的 研究负载日本血吸虫谷胱甘肽S-转移酶(glutathione S-transferase,GST)和可溶性虫卵抗原(soluble egg antigen,SEA)的树突状细胞的表型. 方法 骨髓来源的细胞经白介素-4(interleukin-4,IL-4)、粒细胞-巨噬细胞集落刺激因子(granulocyte-macrophage colony stimulating factor,GM-CSF)诱导培养,获得树突状细胞,体外经GSI、SEA抗原刺激.用异硫氰酸荧光素(fluorescein isothiocyanate,FITC)标记的抗GST单克隆抗体染色法检测GST的负载情况.流式细胞仪检测血吸虫抗原负载后树突状细胞表面CD40、CD80、CD86、CD11c分子的表达情况,并与脂多糖(lipopolysaccharide,LPS)、PBS刺激组作比较. 结果 GST负载后在荧光显微镜下可观察到抗GST的特异荧光,表明抗原已被细胞摄取.与LPS相比较,GST、SEA抗原负载后,树突状细胞表面分子CD40、CD86上调不显著,而更类似于PBS刺激组. 结论 日本血吸虫抗原负载后,树突状细胞的表型类似于未成熟表型.

Phenotype of dendritic cell loaded with antigens from Schistosoma japonicum

Objective To investigate the phenotype of dendritic cell( DC) loaded with GST and SEA from Schistosoma japonicum. Methods Bone marrow cells were cultured in the presence of IL-4 and GM-CSF to induce dendritic cells(DCs). These DCs were stimulated by purified GST and SEA antigen from Schistosoma japonicum, respectively. FITC labeled anti-GST monoclonal antibody was used to detect the loading of antigen. After that, the expression of CD40, CD80, CD86 and CD11c on the membrane of DC were analyzed by fluorescence activated cell sorting(FACS). Results The loading of antigen was confirmed by the detection of GST on the cells. GST was successfully loaded on DCs. The positive rates of CD40, CD80 and CD86 in the groups stimulated with GST or SEA were not raised significantly, comparing to the PBS control. Conclusion DC loaded with GST and SEA antigen from Schistosoma japonicum exhibited an immature phenotype.