5-氮胞苷诱导的心室成纤维细胞移植对心肌梗死后心功能的影响

目的：探讨5-氮胞苷诱导的心室成纤维细胞（CFs）植入大鼠梗死区组织后心功能的变化。方法： 取远交群（SD）乳鼠提取CFs，行体外培养、扩增，取第3代细胞以5-氮胞苷诱导。另取20只SD大鼠随机分为治疗组和对照组各10只，建立大鼠AMI模型，2周后，治疗组将诱导后的CFs注入梗死区及其周边；对照组则注入等量细胞培养液。分别在移植前及移植4周后行心脏UCG及MPA心功能分析系统检测心功能。结果：与移植前及对照组移植4周后比较，治疗组移植4周后的左心室收缩末期内径和容积、左心室压最低值、左心室舒张末压明显降低，每搏量、左心室短轴缩短率、左心室射血分数、左心室压最高值、左心室压力上升的最大变化速率及其对应的左心室压、左心室压力下降的最大变化速率及其对应的左心室压明显增加（均为P〈0.05～0.01）。结论： 5-氮胞苷诱导的CFs植入大鼠心肌梗死区可以改善左心室收缩及舒张功能。

Effects of 5-azacytidine-treated ventricular fibroblasts transplantation on cardiac function following myocardial infarction in rats

Objective： To study the effects of 5-azacytidine-treated ventricular fibroblasts transplantation on cardiac function following myocardial infarction in rats. Methods： Ventricular fibroblasts were obtained from neonatal Sprague-Dawley rats, culture-expanded to third passage, treated with 5-azacytidine in 10 txmol/L concentra- tion. Twenty Sprague-Dawley adult rats were randomly divided into treatment and control group. Acute myocardial infarction models were established by ligating the left anterior descending coronary artery. The cells were implanted into infarcted myocardium and nearby tissue in the treatment group and only medium was infused in the control groups 2 weeks later. Two-dimensional echocardiography and MPA cardiac function analysis system were used to e- valuate the cardiac function 2 and 4 weeks after coronary occlusion. Results： Two-dimensional echocardiography revealed that there was significant decrease in left ventrieular end-systolic diameter （LVDs） , left ventricular endsystolic volume （ LVESV）, minimal left ventricular pressure （Pmin） and left ventricular end diastolic pressure （ LV- EDP） after transplantation. There was significant increase in stroke volume （SV）, fraction shorting （FS）, left ventricular ejection fraction （EF） , maximal left ventricular pressure （Pmax） , maximal descending derivative of left ventricular pressure （dP/dtmax ） and its corresponding left ventricular pressure （P@ dP/dtmax ） , maximal descending derivative of left ventricular pressure （ - dP/dtmax） and its corresponding left ventrieular pressure （P@ - dP/dtmax） in treatment group. Significant differences were also found in LVDs, LVESV, SV, FS, EF, Pmax, P@ dP/dtmax,, dP/dt LVEDP, P@ -dP/dtmax and -dP/dt between two groups after transplantation. Conclusion： Transplantation of 5-azacytidine-treated ventricular fibroblasts improves both left ventricular systolic and diastolic funetions in the infarcted heart.