老年男性CYP17基因多态性与血性激素水平的关系

目的:探讨CYP17基因多态性与老年男性血性激素水平的关系。方法:83例正常男性平均年龄66.7岁,分为<66.7岁组(36例)和>66.7岁组(47例),分别提取他们的血DNA标本。设计引物通过PCR技术扩增出包括CYP17基因多态位点的片段,用限制性内切酶MspA1Ⅰ进行酶切,确定出CYP17基因的3种基因型,即A1/A1、A1/A2、A2/A2,并经测序证实。同时测定血清中睾酮(T)和雌二醇(E2)水平。结果:3种基因型之间的T和E2水平差异均无显著性;年龄>66.7岁组与<66.7岁组比较,T和E2水平差异无显著性,但T/E2比值显著升高(P<0.05)。结论:CYP17基因的多态性并未使男性体内的性激素水平发生显著性差异,随着年龄增加,T水平虽有所下降,但T/E2比值却有显著增加,这可能与老年男性前列腺增生和前列腺癌的发生有密切关系。

Association between Polymorphism of CYP17 Gene and Serum Hormone Concentrations in Aged Men

OBJECTIVE: To investigate the association between polymorphism of CYP17 gene and serum hormone concentrations in aged men. METHODS: Eighty-three healthy men at the average age of 66.7 were divided into a < 66.7 group (n = 36) and a > 66.7 group (n = 47), and the polymorphism of CYP17 gene in the 5' promoter region was investigated by PCR using DNA from the men's peripheral blood lymphocytes. A new recognition site was created for the restriction enzyme MspA1 I by transition (T --> C) in the risk allele (A2). Three genotypes A1/A1, A1/A2, A2/A2 were established, serum sex-hormone levels measured, and mean hormone concentration evaluated in each genotype and age group. RESULTS: No evidence was found that the testosterone (T) level, estrogen (E2) level and T/E2 ratio were associated with the genotype of CYP17 gene. There was no significant difference in T and E2 levels between the two groups, but there was a significant increase in the T/E2 ratio (P < 0.05). CONCLUSION: A2 allele does not increase sex hormone levels in aged men, but the T/E, ratio was higher in the > 66.7 group than in the < 66.7 group. This may be closely associated with the mechanism of benign prostate hyperplasia and prostate cancer in aged men.