GnRHⅡ与GnRH Ⅰa对子宫内膜异位症患者离体间质细胞增殖抑制的影响

目的:探讨Ⅱ型促性腺激素释放激素(gonadotropin-releasing hormoneⅡ,GnRHⅡ)与Ⅰ型促性腺激素释放激素激动剂(gonadotropin-releasing hormoneⅠagonist,GnRH Ⅰa)对子宫内膜异位症(endometriosis,EMs)患者离体培养的子宫内膜间质细胞...

Inhibitory effect of GnRHII and GnRH Ia on the stromal cell proliferation from endometriosis patients

ObjectiveTo investigate the inhibitory effect of gonadotropin-releasing hormone Ⅱ(GnRHⅡ) and gonadotropin-releasing hormoneⅠagonist (GnRH Ⅰa) on the proliferation of endometrial stromal cells in vitro from endometriosis patients. MethodsDifferent concentrations of GnRHⅡ or GnRH Ⅰa were added into the cultured endometrial stromal cells in vitro to detect the cell proliferation inhibition by MTT test. ResultsThe inhibitory rate of GnRHⅡ or GnRH Ⅰa on eutopic and ectopic endometrial stromal cells in vitro was both dose- and time-dependent (P<0.05). Effect of GnRHⅡ or GnRH Ⅰa on the inhibitory rate of ectopic endometrial stromal cells was significantly higher than that of eutopic (P<0.05). GnRH Ⅱhad a higher inhibitory rate on the endometrial stromal cells in vitro than did GnRH Ⅰa (P<0.05).ConclusionGnRHⅡ has more antiproliferative effect on endometrial stromal cells than GnRH Ⅰa in vitro, especially on ectopic endometrial stromal cells, suggesting that GnRHⅡ may be more effective than GnRH Ⅰa on endometriosis.