| 大鼠全脑缺血缺氧损伤后血液中S-100、CK—BB、NSE水平变化的研究 |
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| 引用本文： | 金泓,赖旭东,钟莉. 大鼠全脑缺血缺氧损伤后血液中S-100、CK—BB、NSE水平变化的研究[J]. 医疗保健器具, 2011, 18(1): 19-20. DOI: 10.3936/j.issn.1674-4659.2011.01.0019 |
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| 作者姓名： | 金泓 赖旭东 钟莉 |
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| 作者单位： | 1. 暨南大学第四附属医院/广州市红十字会医院,急诊科,广东广州,510220
2. 暨南大学第四附属医院/广州市红十字会医院,检验科,广东广州,510220 |
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| 摘 要： | 目的探讨大鼠全脑缺血缺氧损伤后血液中S-100蛋白（S-100）、磷酸肌酸激酶脑型同工酶（CK—BB）、神经元特异性烯醇化酶（NSE）的水平变化。方法采用成年SD大鼠全脑缺血缺氧损伤模型：戊巴比妥腹腔注射麻醉后．用4．0双线结扎右侧颈总动脉,恢复4小时后,在37℃恒温低氧箱内,给予吸入8％氧气和92％氮气混合气体2小时。用放射免疫法和琼脂糖凝胶电泳法对对照组、缺氧缺血后6、12、24、48、72小时进行动态观测全脑缺血后血液中S-100、CK—BB、NSE水平的变化。结果在全脑缺血后,与对照组比较,血液和脑脊液中S-100、CK—BB、NSE在24～48小时都有明显增加（P〈0．05）。结论在全脑缺血损伤时血液和脑脊液中S-100、CK—BB、NSE水平都在早期明显升高．提示这三种指标可作为评价大鼠全脑缺血缺氧损伤的早期诊断指标。
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| 关 键 词： | 大鼠 脑缺血 磷酸丙酮酸水合酶 神经组织蛋白质S-100 肌酸激酶同工酶类 |
A Study on the Changes of S-100, CK-BB and NSE Levels in Blood after Hypoxic-ischemic Brain Damage in Rats |
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| JIN Hong,LAI Xudong,ZHONG Li. A Study on the Changes of S-100, CK-BB and NSE Levels in Blood after Hypoxic-ischemic Brain Damage in Rats[J]. Medicine Healthcare Apparatus, 2011, 18(1): 19-20. DOI: 10.3936/j.issn.1674-4659.2011.01.0019 |
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| Authors: | JIN Hong LAI Xudong ZHONG Li |
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| Affiliation: | JIN Hong 1,LAI Xudong 1,ZHONG Li 2(1Department of Emergency,2Clinical Laboratory,the Fourth Affiliated Hospital of Jinan University,Guangzhou Red Cross Hospital,Guangzhou 510220,China) |
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| Abstract: | Objective To analyse the changes of S-100 protein(S-100),creatine phosphokinase BB isoenzyme(CK-BB),and neuron-specific enolase(NSE) levels in blood after hypoxic-ischemic brain damage in rats.Methods The model of hypoxic-ischemic brain damage in postnatal 180 days SD rats.The model was established by ligation of the right common carotid arteries for 4 hrs in rats,and then exposure of the rats to 8% of oxygen and 92% of nitrogen for 2 hrs.The blood samples were taken from 90 rats at the sequential time poin... |
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| Keywords: | Rats Cerebral anoxia Cerebral ischemia Protein S-100 Creatine kinase isoenzymes |
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