| HPLC法测定赤芍中芍药苷的含量 |
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| 引用本文： | 周海玲,许舜军,杨柳.HPLC法测定赤芍中芍药苷的含量[J].医疗保健器具,2011,18(4):487-488. |
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| 作者姓名： | 周海玲 许舜军 杨柳 |
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| 作者单位： | 1. 广州中医药大学第二附属医院,广东,广州,510102
2. 澳门科技大学澳门药物及健康应用研究所,澳门 |
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| 摘 要： | 目的建立赤芍中芍药苷的含量测定方法。方法色谱柱为Waters symmetry C18柱（250 mm×4.6 mm,5μm）;流动相为乙腈（A）-0.1%磷酸（B）线性梯度洗脱,0 min：10%A,5 min：15%A,25 min：22%A,45 min：70%A,46 min：80%A,50 min：80%A;平衡时间15 min;流速：0.8 mL.min-1;进样量20μL;柱温25℃;DAD检测器采集范围：195～400 nm;检测波长：230 nm。结果芍药苷在0.39μg～15.6μg范围内线性关系良好,r2=0.9999,平均回收率为94.8%,RSD=2.7%。结论该法简便,重复性、稳定性良好,可作为赤芍中芍药苷的含量测定方法,对赤芍的质量评价具有重要的参考价值。
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| 关 键 词： | HPLC法 赤芍 芍药苷 含量 测定 |
Determination of the Content of Paeoniflorin in Radix Peony Rubra by HPLC Method |
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| ZHOU Hailing,XU Shunjun,YANG Liu.Determination of the Content of Paeoniflorin in Radix Peony Rubra by HPLC Method[J].Medicine Healthcare Apparatus,2011,18(4):487-488. |
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| Authors: | ZHOU Hailing XU Shunjun YANG Liu |
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| Institution: | ZHOU Hailing 1,XU Shunjun 2,YANG Liu 1(1The Second Affiliated Hospital,Guangzhou University of TCM,Guangzhou 510102,China,2The Institute of Drug and Health Applications in Macau Science and Technology University,Macau,China) |
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| Abstract: | Objective To establish the method for the determination of peoniflorin in the Radix Peony Rubra.Methods The chromato column was Waters symmetry C18 column（250 mm × 4.6 mm,5 μm）;mobile phase was acetonitrile（A）-0.1% phosphoric acid（B） linear gradient elution,0 min： 10% A,5 min： 15% A,25 min： 22% A,45 min： 70% A,46 min： 80% A,50 min： 80% A;equilibrium time：15 min;flow rate： 0.8 mL· min-1;injection volume： 20 μL;column temperature was 25 ℃;DAD detector acquisition range： 195 ~ 400 nm;detection wavelength： 230 nm.Results Paeoniflorin in the range 0.39 μg ~ 15.6 μg was good linear relationship,r2 = 0.9999,the average recovery was 94.8%,RSD = 2.7%.Conclusion The method is simple,reproducible,good stability,and can be used in the determination of paeoniflorin in the Radix Peony Rubra,which has important reference value for quality evaluation of Radix. |
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| Keywords: | HPLC Radix Peony Rubra Paeoniflorin Content Determination |
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