放射免疫沉淀法与酶联免疫吸附试验检测抗-LSP的双盲对照研究

研究表明,抗肝细胞膜特异性脂蛋白抗体(抗-LSP)在病毒性肝炎,特别是慢性活动性肝炎的发病机理中可能起较重要的作用。关于抗-LSP的检测,国内外文献中多

Double blind controlled stuby for detection of anti-LSP by RIA and ELISA

In an attempt to understand further occurrence of anti-LSP and to go further into the causes of different results in various laboratories,using double blind controlled method,the sera from 113 patients with various type of viral hepatitis,cirrhosis and HBsAg chronic carrier were detected for anti-LSP by RIA and ELISA.The results indicated that the frequency of anti-LSP detected by RIA was slightly higher than that by ELISA(P>0.05).The sera of 90 patients were positive or negative detectedsimultaneously by RIA and ELISA,and the concordance rate of both methods was79.7%.The sera of 18 patients were positive detected by RIA and negative by ELISA,and the sera of 5 patients were positive detected by ELISA and negative by RIA,sothe inconsistent rate of both methods were 15.9%and 4.4% respectively.Based onthe literatures reviewed,the authors suggested that the causes of inconsistent resultsin various laboratories might be related to following factors,1.different sensitivitybetween various methods for deermination;2.the diffences between experimental conditions;3.the antigens used in the test from different species;4.the blood specimens collected at different period of the course.