| caspase-3抑制剂对肾缺血再灌注损伤的保护作用 |
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| 引用本文： | 王占坤,孙立江,李旭东.caspase-3抑制剂对肾缺血再灌注损伤的保护作用[J].青岛大学医学院学报,2008,44(2):139-142. |
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| 作者姓名： | 王占坤 孙立江 李旭东 |
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| 作者单位： | 青岛大学医学院附属医院泌尿外科,山东,青岛,266003 |
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| 摘 要： | 目的 观察caspase-3抑制剂对缺血再灌注损伤肾脏的保护作用,并探讨其作用机制.方法 建立小鼠肾缺血再灌注模型,将实验动物随机分为3组:假手术组、缺血再灌注组、治疗组(各10只).治疗组小鼠给予Ac-DEVD-CHO (4 μg/g)0.3 mL,缺血再灌注组再灌注时给予0.3 mL含体积分数0.01 DMSO的生理盐水,假手术组不夹闭肾蒂,余处理同缺血再灌注组.分别检测各组血肌酐(Cr)含量、尿素氮(BUN)含量、细胞凋亡指数(AI)、caspase-3活性、髓过氧化物酶(MPO)活性、黏附分子-1(ICAM-1)表达指数.结果 治疗组与缺血再灌注组比较,血Cr和BUN均明显降低,caspase-3活性受到明显抑制,AI明显下降,MPO活性显著降低,ICAM-1表达明显减少,差异均有显著性(F=36.10～574.13,P<0.05).结论 Ac-DEVD-CHO通过抑制细胞凋亡及炎症反应减轻肾脏缺血再灌注损伤.
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| 关 键 词： | 肾 再灌注损伤 半胱氨酸内肽酶类 细胞凋亡 |
| 文章编号： | 1672-4488(2008)02-0139-04 |
| 修稿时间： | 2007年11月28 |
THE PROTECTION OF caspase-3 INHIBITOR ON RENAL ISCHEMIA-REPERFUSION INJURY |
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| WANG ZHAN-KUN,SUN LI-JIANG,LI XU-DONG.THE PROTECTION OF caspase-3 INHIBITOR ON RENAL ISCHEMIA-REPERFUSION INJURY[J].Acta Academiae Medicinae Qingdao Universitatis,2008,44(2):139-142. |
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| Authors: | WANG ZHAN-KUN SUN LI-JIANG LI XU-DONG |
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| Institution: | WANG ZHAN-KUN,SUN LI-JIANG,LI XU-DONG(Department of Urology Affiliated,The Affitiated Hospital of Qingdao University Medical College,Qingdao 266003,China) |
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| Abstract: | Objective To study the protection and its mechanisms of caspase-3 inhibitor on renal ischemia-reperfusion injury. Methods A renal ischemia-reperfusion model was made in 30 rats,which were evenly divided into three groups,i.e.sham-operation group(S-O group),ischemia-reperfusion(I-R group),and Ac-DEVD-CHO group(treated group).Ac-DEVD-CHO was given to treated group;normal saline,0.3 mL,to I-R group while reperfusion being done;for S-O group,no renal pedicles were clamped.The myeloperoxidase(MPO) and caspase-3 ... |
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| Keywords: | Kidney Reperfusion injury Cysteine endopeptidases Apoptosis |
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