重组质粒pZeoSV2（＋）／CpG—HBcAg（ISS）对BALB／c小鼠的免疫作用

目的：探讨重组质粒pZeoSV2（＋）／CpG-HBcAg（ISS）对BALB／c小鼠免疫的作用。方法：构建真核表达质粒pZeoSV2（＋）／CpG-HBcAg（ISSb，c），将其免疫BALB／c小鼠，ELISA法检测免疫后小鼠血清中IFN-γ、IL-2、IL-12、IL-4和IL-10的含量。结果：pZeoSV2（＋）／CpG-HBcAg（ISSb）组较pZeoSV2（＋）／CpG-HBcAg（ISSc）组免疫BALB／c小鼠后能使Th1型细胞因子IFN-γ、IL-2和IL-12的表达增强，抑制Th2型细胞因子IL-4和IL-10的产生。结论：含CpG-HBcAg（ISSb）的重组质粒能够明显诱导小鼠血清Th1型细胞因子的表达，抑制Th2型细胞因子的表达。

Immune Effects of Recombinant Plasmid pZeoSV2(+)/CpG-HBcAg(ISS) on BALB/c Mouse

Objective： To explore the immone effects of recombinant plasmid pZeoSV2（ ＋ ）/CpG-HBcAg（ISS） on BALB/c mice. Methods： Recombinant plasmid pZeoSV2（ ＋ ）/CpG-HBcAg（ISSb, c） was constructed and was used to immune BALB/c mice. The serum IFN-γ, IL2, IL-12, IL-4 and IL-10 level were detected by ELISA. Resuits： The IFN-γ, IL2 and IL-12 induced by pZeoSV2 （ ＋ ）/CpG-HBcAg （ISSb）group were obviously higher than those of pZeoSV2 （＋）/CpG-HBcAg（ISSc）group （P（0. 01）, as well as , levels of IL-4 and IL-10 induced by pZeoSV2（ ＋ ）/CpG-HBcAg（ISSb）group were obviously less than those of pZeoSV2（ ＋ ）/CpG-HBcAg（ISSc）group （P〈0. 01）. Conclusions： The result showed that high levels of Thl type cytokines including IL-2, IFN-γ and IL-12 were induced in pZeoSV2（ ＋ ）/CpG-HBcAg（ISSb）recombinant plasmid groups, where as the levels of Th2 type cytokines such as IL-4 and IL-10 were inhibited obviously.