不同类型宫颈标本人乳头瘤病毒感染及亚型分布

目的探讨不同类型宫颈标本(指宫颈细胞刷标本和宫颈环切标本)人乳头瘤病毒(HPV)感染及亚型分布。方法采用HPV通用引物对482例宫颈组织标本中可能存在的HPV基因进行PCR扩增,对扩增产物采用导流杂交基因芯片技术进行HPV分型。结果 482例标本共检出HPV阳性124例,总感染率为25.73%(124/482),21种亚型共检出18种,低危型42、43、44未检出。宫颈细胞刷标本组267例中共检出HPV阳性97例,感染率为36.33%;宫颈环切标本组215例中共检出HPV阳性27例,感染率为12.56%,两组差异有统计学意义(χ2=35.22,P<0.01)。宫颈细胞刷标本组多重感染率(包括同时感染两种或两种以上不同亚型)为9.36%(25/267),宫颈环切标本组多重感染率为1.86%(4/215),两组差异有统计学意义(χ2=11.86,P<0.01)。结论宫颈细胞刷标本和宫颈环切标本HPV检出率存在统计学意义,临床在采取标本时应尽可能采集宫颈细胞刷标本,以防止漏检;HPV亚型分布存在一定的地域差异,针对不同地区不同亚型的疫苗研究,将是防治宫颈癌的一个重要方向。

Human papillomavirus infection and their subtypes distribution in different cervical specimens

Objective To investigate the status of human papillomavirus（HPV） infection and subtypes distribution in different cervical specimens （cervical brush specimens and cervical loop excision specimens）. Methods To use general primer of HPV to PCR amplificate genes of HPV which may exist in 482 cases of cervical specimens,then to selectthe products for HPV type analysis using flow-through hybridization. Results From 482 cases, 124 cases were infected with HPV, the total infection rate was 25. 73% （124/482）, 18 kinds subtypes were found in all 21 kinds,the low-risk kinds of 42,43,44 were nol found. In 267cases of cervical brush specimens, 97 cases were tested positive with the postive rate of 36.33%,in 215 cases of cervical loop excision specimens,27cases were tested positive with the postive rate of 12. 56%/00, showing statistical difference between them （9（2 = 35. 22, P~0. 01）. In cervical brush specimens,the multiple infection rate （simultaneous infection with two subtypes and more） was 9. 36% （25/ 267） ,but in loop excision specimens of cervical, the multiple infection rate was 1.86% （4/215） also with statistical difference （9（2 11.86,P~0.01）. Conclusion The detection rate between cervical brush specimens and loop excision specimens of cervical is different in statistics,so we should collect cervical brush specimens in clinic to avoid undetec tion：the distribution in HPV subtypes is different in different areas, so the research of vaccine should aimed at different subtypes in different areas, which can become an important direction in prevention of HPV.