计算机辅助精液分析系统的性能评价

目的了解计算机辅助精液分析系统(CASA)的性能。方法选择精子密度作为主要指标,对CA-SA检测的线性、批内精密度、干扰试验、与手工计数的比较,以及操作人员之间比对进行了初步评价。结果线性实验结果:精子密度在(5~100)×106/mL范围内相关系数r2=0.999 1,批内精密度为4.04%,白细胞的影响度为2.03%~2.92%,与手工比对的P值为0.52,操作人员之间比对P值为0.000 65。结论 CASA在精子密度(5~100)×106/mL范围内有良好的线性,有较高的批内精密度和较强的抗生物干扰物质的能力,与手工法比较差异无统计学意义,但操作人员间比对差异有统计学意义。

The evaluation of the performance of computer-aided semen analysis system

Objective To understand the performance of computer-aided semen analysis（CASA） system.Methods We selected the sperm density as the main parameter,and compared the results of the linearity tested by CASA,intraassay precision,interference test with those of manual counting,and compared the results from different operators as a preliminary evaluation.Results When the sperm density was between（5-100）×10^6/mL,the linear correlation coefficient result was r^2=0.999 1,With intraassay precision 4.04%.The degree of white blood cells interference was between 2.03%-2.92%,and P value of the comparison of the result of CASA and manual counting was 0.52.P value of the comparison of the results between different operators was 0.000 65.Conclusion When the sperm density is between（5-100）×10^6/mL,CASA can get a good linear result,and have better intraassay precision,a better anti-biotic substances ability.The difference of results between the CASA and manual counting is not significant,but the difference of results between different operators is significant.