不同保存温度对精浆天门冬氨酸氨基转移酶活性和稳定性的影响

目的探讨不同保存温度对精浆天门冬氨酸氨基转移酶(AST)活性和稳定性的影响。方法将10份精浆样本分别保存在室温、4、-20、-80℃等4种不同温度条件下,分别于第1、2、3、5、7、9天测定精浆AST,观察其活性和稳定性的变化。结果在-20℃和-80℃温度条件下精浆AST活性的天间比较差异无统计学意义(P>0.05);在4℃温度条件下精浆AST活性的天间比较差异无统计学(P>0.05),但精浆AST活性从第5天开始呈上升趋势;在室温条件下精浆AST活性从第3天开始比较差异有统计学意义(P<0.05),从第2天开始呈下降趋势。结论在-20℃和-80℃温度条件下精浆AST的活性比较稳定。

The influence of different temperature on the activity and stability of seminal plasma AST

Objective To explore the influence of different temperature on the activity and stability of seminal plasma aspartate aminotransferase（AST）.Methods 10 seminal plasma samples were stored at room temperature,4 ℃,-20 ℃ and-80 ℃,we tested the content of seminal plasma AST after 1,2,3,5,7,9 days,respectively.And analyzed the activity and stability change of seminal plasma AST.Results The activity of seminal plasma AST between days was not significantly different（P0.05）at-20 ℃ and-80 ℃.The activity of seminal plasma AST between days was not significantly different（P0.05）at 4 ℃,but the activity of seminal plasma AST started increasing form the fifth day.The activity of seminal plasma AST stored at room temperature started changing from the third day,（P0.05）,it started decreasing form the next day.Conclusion The activity of seminal plasma AST is relatively stable at-20 ℃ and-80 ℃.