FT-2 antigen for distinguishing lymphocyte subpopulations in the developing thymus |
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Authors: | T Takashi M Kasai N Tanaka T Takahashi T Tokunaga |
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Institution: | 1. Department of Cellular Immunology, National Institute of Health, Shinagawa-ku, Tokyo 141, Japan;2. Research Institute, Daiichi Seiyaku Co., Ltd., Edogawa-ku, Tokyo 134, Japan;3. Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Nagoya 464, Japan |
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Abstract: | The surface phenotypes of lymphoid cells in the developing embryonic thymus were characterized by using monoclonal antibodies. FT-2 antigen thus defined was predominantly expressed on thymocytes in the earlier embryonic stages in all the inbred mouse strains tested. The immunofluorescence and immunoperoxidase tests indicated that, like FT-1 antigen, the proportion of FT-2+ fetal thymocytes rapidly decreased with increase in gestation time, and these cells disappeared by day 19 of gestation. The treatment of fetal thymocytes with anti-FT-1 plus complement eliminated not only FT-1+, but also FT-2+ cells, whereas the treatment with anti-FT-2 failed to eliminate approximately 40% of FT-1+ cells, suggesting that embryonic thymocytes can be provisionally divided into at least three subpopulations, FT-1+2+, FT-1+2- and FT-1-2-. |
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Keywords: | fetal thymus antigen-1 fetal thymus antigen-2 thymocyte subpopulations T cell differentiation |
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