Genetic and cytological characterization of the RecA-homologous proteins Rad51 and Dmc1 of Schizosaccharomyces pombe |
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Authors: | Grishchuk Alexandra L. Kraehenbuehl Rolf Molnar Monika Fleck Oliver Kohli Juerg |
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Affiliation: | (1) Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland |
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Abstract: | The Schizosaccharomyces pombe rad51+ and dmc1+ genes code for homologues of the Escherichia coli recombination protein RecA. Deletion of rad51+ causes slow growth, retardation of cell division and a decrease in viability. rad51 cells have a defect in mating-type switching. The DNA modification at the mating-type locus required for mating-type switching contributes to slow growth in the rad51 mutant. Cell mating is reduced in crosses homozygous for rad51. Ectopic expression of the dmc1+ gene allowed us to demonstrate that the reduction in meiotic recombination in dmc1 mutants is not caused by a disturbance of rad24 expression from the dmc1-rad24 bicistronic RNA. We describe the functional defects of terminally epitope-tagged Dmc1 and Rad51 and discuss it in terms of protein interaction. Presumptive Rad51 and Dmc1 foci were detected on spreads of meiotic chromatin. |
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Keywords: | Rad51 Dmc1 Slow growth Meiotic recombination |
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