| 血链球菌丙酮酸氧化酶基因表达克隆的构建与鉴定 |
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| 引用本文： | 侯本祥,章锦才,等.血链球菌丙酮酸氧化酶基因表达克隆的构建与鉴定[J].华西医科大学学报,2001,32(1):15-17. |
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| 作者姓名： | 侯本祥 章锦才 |
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| 摘 要： | 目的 构建血链球菌丙酮酸氧化酶基因Sopox的表达质粒，为研究丙酮酸氧化酶基因Sopox表达的调节奠定基础。方法 将从血链球菌ATCC10557克隆的丙酮酸氧化酶基因Soopox重组到pBV220，表达载体，并转化入大肠杆菌E.coliJM105，观察Sopox基因在大肠杆菌中的表达。结果 通过酶切后电泳鉴定，Sopox基因重组入pBV220并转化入E.coliJM105；经42℃yte nf rg ,SDS-PAGE显示pBV220/Sopox/JM105表达分子量约为65kd的蛋白，与根据Sopox基因DNA序列预测的蛋白分子量一致；pBV220/Sopox/JM105的蛋白表达在42℃诱导4小时后达高峰。结论 丙酮酸氧化酶基因Sopox已被成功地克隆到pBV220/Sopox/JM105的蛋白表达在42℃诱导4小时后达高峰。结论 丙酮酸氧化酶基因Sopox已被成功地克隆到pBV220，并在E.coliJM105中表达。
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| 关 键 词： | 丙酮酸氧化酶 血链球菌 基因表达 克隆 质粒 牙周病 |
Expression of pyruvate oxidase gene sopox from Streptococcus sanguis in E. coli] |
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| B Hou,J Zhang,R Zhang,Y Zhang.Expression of pyruvate oxidase gene sopox from Streptococcus sanguis in E. coli][J].Journal of West China University of Medical Sciences,2001,32(1):15-17. |
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| Authors: | B Hou J Zhang R Zhang Y Zhang |
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| Institution: | Department of Periodontology, School of Stomatology, WCUMS, Chengdu 610041, China. |
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| Abstract: | OBJECTIVE: To reconstruct the expression plasmid of Sopox gene for further understanding the regulation of its expression. METHODS: Sopox was recombined with expression vector pBV220 and the expression of Sopox in E. coli JM105 was observed after transformation. RESULTS: pBV220/Sopox/JM105 expressed a protein with molecular weight of 65 kd on SDS-PAGE after induction, and the expression reached the maximal amount with induction at 42 degrees C for 4 hours. CONCLUSION: Sopox was successfully cloned into pBV220 and expressed in E. coli JM105. |
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