交泰丸对2型糖尿病大鼠胰腺脂肪沉积和胰岛细胞凋亡的影响

该研究采用小剂量链脲佐菌素（STZ）尾静脉注射和高脂饮食8周建立大鼠T2DM模型，给予交泰丸治疗，检测葡萄糖耐量试验（OGTT）、空腹胰岛素（FINS）、游离脂肪酸（FFA）、血脂，计算胰岛素抵抗指数（HOMA-IR），胰腺组织病理学检查，脂质抽提法检测胰腺组织甘油三酯（TG）含量，原位末端脱氧核糖核苷酸转移酶介导dUTP标记法（TUNEL）法检测胰岛细胞凋亡情况。结果显示，模型组OGTT异常，FINS，HOMA-IR，FFA升高，血脂紊乱，胰腺组织见明显脂肪沉积，胰腺组织TG含量明显升高，胰岛细胞凋亡增加；与模型组比较，交泰丸组OGTT改善，FINS，HOMA-IR，FFA下降，血脂紊乱好转，胰腺组织脂肪沉积减少，胰腺组织TG含量明显下降，胰岛细胞凋亡减少。综上所述，交泰丸可有效治疗大鼠T2DM，其机制可能与减少胰腺脂肪沉积和胰岛细胞凋亡有关。

Effect of Jiaotai pill on pancreatic fat accumulation and islet cell apoptosis in rats with type 2 diabetes

In this study, the rat type 2 diabetes mellitus(T2DM) model was established through tail vein injection with low dose of streptozotocin(STZ) and high fat diet for 8 weeks, and then treated with Jiaotai Pill. The oral glucose tolerance test(OGTT), fasting serum insulin(FINS), free fatty acid(FFA) levels and blood lipid were assayed. HOMA-IR was calculated. Pancreatic pathology was performed. And pancreatic triglyceride(TG) content was examined by the lipid extraction method. Pancreatic islet cell apoptosis were detected by terminal dexynucleotidyl transferase(TdT)-mediated dUTP nick end labeling(TUNEL). According to the results, the model group showed abnormal OGTT, increased FINS, HOMA-IR, FFA, lipid disorder, obvious fat accumulation and significantly increased TG content in pancreatic tissues, and enhanced pancreatic islet cell apoptosis. Compared with the model group, the Jiaotai Pill group displayed improved OGTT, reduced FINS, HOMA-IR, FFA, recovered lipid disorder, decreased fat accumulation and significantly declined TG content in pancreatic tissues, and lowered pancreatic islet cell apoptosis. In summary, Jiaotai pill could effectively treat type 2 diabetes in rats. Its mechanism may be related to the reduction in pancreatic fat accumulation and islet cell apoptosis.