新西兰狼疮鼠抗染色质抗体易感基因染色体定位研究

目的确定新西兰小鼠自身抗染色质抗体的遗传易感基因染色体定位.方法建立新西兰黑色品系(NZB)与新西兰白色品系(NZW)F1×NZB回交小鼠模型.采用覆盖小鼠19条染色体的微卫星遗传标记及数量性状位点(QTL)分析进行基因定位.结果确定了自身抗染色质抗体产生的两个遗传易感基因,一个位于NZW小鼠第9染色体中段30厘摩临近;另一个易感基因位于NZW小鼠第17染色体近中心端19厘摩处(Lods值＞3).结论(NZB×NZW)F1小鼠自身抗染色质抗体的产生受多基因调控,候选易感基因不仅来自于NZB,也来自于NZW.

Study of susceptibility genes mapping for anti-chromatin antibody in SLE model-New Zealand mice

Objective: To map the susceptibility allele of anti-chromatin antibody in SLE model-(NZB ×NZW)F1 mice. Methods: New Zealand Black (NZB) ×New Zealand (NZW) F1×NZB backcross mice model was set up and polymorphic microsatellite markers and quantitative trait locus (QTL) analysis was used. Results: We got two susceptibility alleles, one was susceptibility allele of anti-chromatin antibody localized on the mesomere about 30 cM of chromosome 9 in NZW; the other was localized on the center about 19 cM of chromosome 17 in NZW too (Lods＞3).Conclusion: Susceptibility allele of anti-chromatin antibody in (NZB×NZW) F1 mice was comprised of multiple genes and the candidate susceptibility genes were derived from both NZB and NZW strain.