自身免疫性前列腺炎大鼠模型的建立

目的:使用前列腺蛋白提纯液建立自身免疫性前列腺炎大鼠模型。方法:选用36只Wistar大鼠,随机分为对照组、低浓度组和高浓度组,每组12只。对照组注射生理盐水,低浓度和高浓度组实验大鼠于0、14 d分别注射等剂量15 mg/ml及80 mg/ml浓度的前列腺蛋白提纯液,4周后处死大鼠,前列腺组织HE染色,取大鼠外周血检测血清炎症因子IL-8、IL-10,血清免疫球蛋白IgA、IgM,辅助性T细胞Th1/Th2等指标。结果:高浓度组有3只大鼠死亡,对照组与低浓度组均无死亡。前列腺大体观察对照组无明显变化,低浓度组大鼠前列腺体积增大,质地稍硬,高浓度组大鼠前列腺质地坚硬,与周围组织粘连。实验组病理切片显示前列腺组织腺体结构破坏,有炎性细胞浸润。大鼠血清IL-8指标低浓度组(129.07±11.48)pg/ml]、高浓度组(147.58±17.70)pg/ml]与对照组(94.12±7.04)pg/ml]比明显升高(P0.05);大鼠血清IL-10指标低浓度组(227.14±18.19)pg/ml]、高浓度组(187.14±16.32)pg/ml]与对照组(252.48±21.72)pg/ml]相比显著降低(P0.05)。大鼠血清IgA与对照组(0.19±0.14)mg/ml]相比,低浓度组(0.25±0.37)mg/ml]和高浓度组(0.31±0.42)mg/ml]明显升高(P0.05);大鼠血清IgM指标低浓度组(0.23±0.41)mg/ml]、高浓度组(0.34±0.58)mg/ml]与对照组(0.17±0.33)mg/ml]相比,显著升高(P0.05)。外周血辅助性T细胞Th1/Th2未见明显改变。结论:低、高浓度组大鼠造模均获成功。使用低浓度的前列腺蛋白提纯液造模的动物死亡率低,病理改变及血清炎症因子变化符合自身免疫性前列腺炎的表现,可作为制备自身免疫性前列腺炎的可靠模型浓度。

Establishment of a rat model of autoimmune prostatitis with purified prostatic proteins

Objective： To establish a rat model of autoimmune prostatitis using purified prostatic proteins （PPP）. Methods： Thirty-six male Wistar rats were randomized into three groups of equal number to receive intramuscular injection of normal saline （ normal control group） and PPP at 15 mg/ml （low-concentration group） and 80 mg/ml （high-concentration group）. At 4 weeks after modeling, the rats were sacrificed for HE staining of the prostate tissue and examination of the inflammatory factors IL-8 and IL-10 in the serum, immunoglobulins IgA and IgM, and regulatory T cells Th1/Th2. Results： Three rats died in the high-concentration PPP group but none in the low-concentration PPP and normal control groups. Gross observation of the prostate showed increased volume and hard texture of the prostate in the two PPP groups, but no significant change in the normal controls. Pathological examination exhibited morphological damage to the prostatic tissue and inflammatory cellular infiltration in the experimental rats. The serum level of IL-8 was significantly higher in the low- and high-concentration PPP groups （ 129.07 ± 11.48] and 147.58 ± 17.70] pg/ml） than in the control （ 94.12 ±7.04] pg/ml） （ P 〈0.05）, while that of IL-10 was remarkably lower in the former two groups （ 227.14 ±18.19] and 187.14± 16.32 ] pg/ml） than in the latter （ 252.48 ±21.72 ] pg/ml, P 〈 0.05）. The serum level of IgA was markedly elevated in the low- and high-concentration PPP groups as compared with that in the control （0.25 ± 0.37] and 0.31 ±0.42 ] vs 0.19±0.14] mg/ml, P〈0.05）, and so was that of IgM （0.23 ±0.41] and 0.34±0.58] vs 0. 17 ±0.331 mg/ml, P〈 0.05）. No significant changes were observed in the levels of regulatory T cells Thl/Th2. Conclusion ： Both low and high concentrations of purified prostatic proteins can be used for the construction of autoimmune prostatitis models in rats, while low concentration is preferable for its advantages of lower mortality of the rats and inducement of more consistent manifestations of autoimmune prostatitis. Natl J Androl, 2014, 20 （5）： 414-418