透明质酸受体精子的质量特性研究

目的:分析具有透明质酸受体精子的质量特征,寻找精子质量评估新指标。方法:用透明质酸包被载玻片检测活动精子-透明质酸结合率,分析其与精液常规、精子膜功能、精子受精功能和精子成熟障碍指标的关系。结果:头部具有透明质酸结合位点的精子,顶体完整性(95.4±3.9)%]、线粒体膜高电位率(97.8±2.1)%]明显高于原始活动精子(68.8±6.2)%和(72.8±7.4)%,P均0.01];精子-透明质酸结合率与多项精液常规参数呈弱相关性(r=0.195~0.268,P均0.05),与诱发顶体反应率和精子正常形态率呈正相关(r=0.666、0.417,P均0.01),与精子核蛋白不成熟度、DNA碎片率和过量残留胞质率呈负相关(r=-0.266、-0.308、-0.218,P0.01、0.01、0.05)。结论:头部存在透明质酸受体的精子,其质膜结构、受精潜能、成熟度俱佳;用于检测精子透明质酸受体的精子-透明质酸结合试验,是独立的精子多重质量评价新指标。

Quality characteristics of human spermatozoa with hyaluronic acid receptors

Objective： To analyze the quality characteristics of human spermatozoa with hyaluronic acid （HA） receptors and search for a new indicator for the assessment of sperm quality. Methods ： Using sperm-HA binding assay with HA-coated slides, we determined the binding rate of motile sperm with HA receptors and analyzed its correlation with routine semen parameters, sperm mem- brane function, sperm fertilizing function and diminished/arrested sperm maturation. Results： The motile sperm with HA binding sites in the acrosomal region showed significantly higher acrosomal integrity （ 95.4 ± 3.9 ] % ） and mitochondrial membrane potential （MMP） （ 97.8 ± 2.1 ] % ） than those in the initial semen （ 68.8 ± 6.2 ] % and 72.8 ± 7.4 ] % ） （ P 〈 0.01 ）. The sperm-HA binding scores were correlated mildly with many routine semen parameters （ r = 0. 195 - 0.268, P 〈 0.05 ）, positively with the acrosome reaction level after ionophore challenge （ r = 0.666, P 〈 0.01 ） and normal sperm morphology （ r = 0.417, P 〈 0.01 ）, and negatively with sperm nucleoprotein immaturation （ r = - 0.266, P 〈 0.01 ）, DNA fragmentation （ r = - 0. 308, P 〈 0.01 ） and excessive re.,ddual cytoplasm （ r = - 0. 218, P 〈 0.05）. Conclusion ： Sperm with HA receptors in the acrosomal region exhibit significant ad- vaJntages in plasma membrane structure, fertilizing potential and maturation. The sperm-HA binding assay, which is based on a relationship between sperm receptors for zona pellucida and HA, is likely to become a new independent indicator for assessing the multiple qualities of spermatozoa.