藏药鲁堆多吉水提物对雄性幼年大鼠生殖系统的影响

目的:研究藏药鲁堆多吉对雄性幼年大鼠生殖系统的影响。方法:将32只SD雄性幼年大鼠分为对照组、鲁堆多吉水提物低、中、高剂量组,灌胃2周后处死,用放射免疫法检测大鼠血清中睾酮(T)的含量,用ELISA试剂盒测量大鼠血清中LH和FSH的含量,称量大鼠的睾丸重量,计算体重增加量、睾丸脏器系数和精子浓度。将高剂量组大鼠血清在57℃水浴锅中灭活后,按0(对照组)、10%、15%、20%的体积分数加到培养的大鼠睾丸间质细胞中,分别用台盼蓝染色法和MTT检测细胞活率和活力,用放免法检测睾丸间质细胞培养液中T的含量,用大鼠环磷酸腺苷(cAMP)ELISA试剂盒检测睾丸间质细胞内cAMP的变化。结果:与对照组相比,低、中、高剂量的鲁堆多吉水提物能使幼年雄性大鼠的血清T水平(1.22±0.32)、(1.06±0.29)、(0.57±0.18)nmol/L vs(3.09±0.42)nmol/L,P0.01]、睾丸重量(0.96±0.09)、(0.92±0.11)、(0.91±0.08)g vs(1.40±0.16)g,P0.01]和精子浓度(0.19±0.07)、(0.17±0.08)、(0.16±0.07)×106/ml vs(1.03±0.16)×106/ml,P0.01]显著降低,并且呈剂量依赖性。与对照组相比,中、高剂量给药组血清中的LH(14.69±0.12)、(14.93±0.28)ng/L vs(13.62±0.89)ng/L,P0.01]、FSH(4.77±0.23)、(4.89±0.38)IU/L vs(4.32±0.18)IU/L,P0.05]显著上升;睾丸脏器系数(51.39±3.09)、(52.28±4.86)、(54.13±6.06)mg/10 g vs(42.22±3.02)mg/10 g,P0.01]显著提高。与对照组相比,鲁堆多吉水提物含药血清能显著抑制睾丸间质细胞内cAMP水平(4.39±0.06)、(4.28±0.07)、(4.11±0.10)nmol/L vs(5.51±0.12)nmol/L,P0.01]和T的合成(1.42±0.15)、(1.12±0.18)、(0.88±0.21)nmol/L vs(1.85±0.18)nmol/L,P0.01]。结论:鲁堆多吉水提物可能通过PKA途径抑制大鼠睾丸间质细胞中T的合成,从而抑制睾丸发育和精子生成,影响雄性幼鼠生殖系统的发育。

Water extract from Codonopsis thalictrifolia Wall affects the reproductive system of male infant rats

Objective： To study the impact of the water extract from Codonpsis thalictrifolia Wall （CTW） on the reproductive system of male infant rats. Methods ： We divided 32 male SD infant rats into four groups of equal number to be treated intragastrically with distilled water （control） and CTW at 10 g/kg （low dose）, 20 g/kg （medium dose）, and 40 g/kg （high dose）, respectively, twice a day for 2 weeks. Then we killed the rats, measured the levels of testosterone （T） , luteinizing hormone （LH） and folliclestimulating hormone （FSH） in the serum, obtained the testis weight, body weight, testis visceral coefficient and sperm concentration, and detected sperm viability, sperm motility and the level of cyclic adenosine monophosphate （cAMP） in the Leydig cells, followed by analysis of differences among different groups using the SPSS software. Results ： Compared with the control group, the low-dose, me- diumdose and highdose CTW groups showed significant decreases in the serum T level （ 3.09 ± 0.42 ] vs 1.22± 0.32 ], 1.06± 0.29] and 0.57±0.18] nmol/L, P〈0.01）, testis weight （1.40±0.16] vs 0.96±0.09], 0.92 ±0. 11] and 0.91 ± 0.08] g, P〈0.01）, and sperm concentration （ 1.03 ±0.16] vs 0.19 ±0.07], 0.17 ±0.08] and 0.16 ±0.07] × 10^6/ml, P 〈0.01 ）, but a dramatic elevation in the testis visceral coefficient （42.22 ± 3.02 ] vs 51.39 ± 3.09 ], 52.28 ± 4.86 ] and 54.13 ±6.06] mg/10 g, P 〈0.01 ） ; the mediumand highdose CTW groups exhibited remarkable increases in the levels of serum LH （13.62 ±0.89] vs 14.69±0.12] and 14.93±0.28] ng/L, P〈0.01） and FSH （4.32±0.18] vs 4.77±0.23] and 4.89 ± 0.38 ] IU/L, P 〈 0.05） ; all the three CTW groups showed markedly inhibited serum T secretion （ 1.85± 0.18 ] vs 1.42 ±0.15], 1.12±0.18] and 0.88 ±0.21] nmol/L, P〈0.01） and intracellular cAMP （5.51 ±0.12] vs 4.39±0.06], 4.28 ± 0.07 ] and 4.11 ± 0. 10 ] nmol/L, P 〈 0.01 ） in the Leydig cells. C