微囊化异种嗅球组织移植联合L-NAME治疗脊髓损伤的实验研究

目的探讨微囊化异种嗅球组织移植联合L-NAME对脊髓继发性损伤的治疗保护作用及机制。方法健康SD大鼠,随机分为对照组、微囊化兔嗅球组织移植组、微囊化异种嗅球组织移植联合L-NAME治疗组,损伤组又设1、3、7、14、28d 5个时相点,紫外分光光度计检测脊髓伊文氏蓝含量,干湿重法测定脊髓组织含水量;免疫组织化学技术检测MMP-9的表达;神经功能评分法（BBB法）评价大鼠术后运动功能的恢复情况。结果脊髓损伤后脊髓伊文氏蓝含量增加、脊髓血管源性水肿、MMP-9表达上调,大鼠的运动功能缺失,经微囊化异种嗅球组织移植联合L-NAME治疗后血脊髓屏障通透性降低、水肿明显减轻、MMP-9表达下调,大鼠的运动功能恢复。结论微囊化异种嗅球组织移植对脊髓继发性损伤有较好的疗效,联合应用嗅球组织细胞和L-NAME在脊髓损伤修复治疗中具有协同作用。

Experimental study on exnotransplantation of microencapsulated olfactory bulb combined with L-NAME for the treatment of spinal cord injury

Objective To explore the protective effects and mechanism of exnotransplantation of microencapsulated olfactory bulb combined with L-NAME in rats at the early stages of spinal cord injury.Methods Healthy SD rats were divided into three groups as following： injury control group,microencapsulated transplantation of rabbit olfactory bulb cells group,microencapsulated transplantation of rabbit olfactory bulb cells combined with L-NAME group,the later two groups were quantified at 1,3,7,14,and 28 d post spinal cord injury.Spinal Evans blue content was examined with the ultraviolet spectrophotometer;Spinal cord water content was detected by caculating dry/wet weight.The change of MMP-9 expression after spinal cord injury was determined by immunohistochemical method.BBB score was designed to assess its behavioral ability.Results Evans blue content and expression of MMP-9 were significantly were significantly increased,vasogenic spinal cord edema,and loss of motor function in rats after spinal cord injury.Permeability of blood-spinal barrier and vasogenic spinal cord edema,MMP-9 were decreased,motor function was recoveried in rats after they were treated with microencapsulated transplantation of rabbit olfactory bulb cells combined with L-NAME.Conclusion Exnotransplantation of microencapsulated strategy of olfactory ensheating cells was effective in the repair of spinal cord injury in some extent.There may be a synergistic effect between olfactory ensheating cells and L-NAME.