Protein delivery of caspase-3 induces cell death in malignant C6 glioma,primary astrocytes and immortalized and primary brain capillary endothelial cells |
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Authors: | Birgit?Zassler Ingolf?E?Blasig Email author" target="_blank">Christian?HumpelEmail author |
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Institution: | (1) Laboratory of Psychiatry, University Clinic of Psychiatry, Innsbruck, Austria;(2) Institute of Molecular Pharmacology, Berlin, Germany;(3) Department of Psychiatry, Laboratory of Psychiatry, University Clinic of Psychiatry, Anichstr.35, A-6020 Innsbruck, Austria |
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Abstract: | Most brain tumors consist of transformed glia cells and are highly vascularized by capillary endothelial cells. The aim of the present study therefore was to deliver pro-apoptotic caspase-3 into malignant C6 glioma and immortalized rBCEC4 brain endothelial cells to induce cell death. Both cell lines were transfected with a reporter protein (-galactosidase) using lipid-mediated gene transfer (FuGENE6TM) or using the novel protein delivery reagent BioPORTERTM. -Galactosidase protein was successfully delivered into both cells, the protein expression peaked around day 2 and was transient. Delivery of caspase-3 induced TUNEL-positive cell death of both cell types. As a control, caspase-3 was also delivered to non-neoplastic primary astrocytes and endothelial cells and induced cell death. In conclusion BioPORTERTM-protein delivery of pro-apoptotic molecules may provide a potent tool to cause death of the cells in brain tumors, however, this method is limited due to its toxicity to non-malignant cells. |
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Keywords: | BioPORTERTM brain tumor caspase-3 C6 FuGENE6TM rBCEC4 |
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