西红花常见伪品的HPLC-DAD鉴别和掺伪量测定

目的:建立HPLC-DAD方法,一次性鉴别西红花常见伪品红花、菊花,并测定其掺伪量。方法:采用高效液相色谱法,流动相为乙腈-0.3%磷酸水;波长范围200～410 nm;羟基红花黄色素A检测波长402 nm,绿原酸检测波长327 nm;梯度洗脱。结果:通过HPLC分析,确定羟基红花黄色素A、绿原酸分别为红花、菊花的特征成分,测定样品含量,可鉴别出样品中是否掺入了红花、菊花,并确定伪品掺入量。羟基红花黄色素A 0.005～0.25 mg.mL-1,绿原酸0.001～0.05 mg.mL-1范围内线性关系良好,方法回收率高,重复性好。结论:此方法能一次性鉴别西红花常见伪品红花、菊花,并测定其掺伪量,对其它药材的相关研究也有参考价值。

Identification and Quantification of Common Adulterants of Saffron (Crocus sativus) by HPLC-DAD

This study was aimed to develop a method to identify and quantify common adulterants of saffron by simultaneously determining specific constituents by HPLC-DAD. The separation was achieved using a C18 column and CH3CN-0.3%H3PO4 solution as a mobile phase with gradient elution. Hydroxysafflor yellow A and chlorogenic acid, used as specific constituents, were detected at 402 nm and 327 nm, respectively. By detecting specific constituents, we can find out if samples were adulterated with safflower and/or chrysanthemum and calculate the percentage. The method was validated in terms of linearity, precision, accuracy and recovery. And results were found to be satisfactory. This method is simple, sensitive and operational in the identification and quantification of common adulterants of saffron.