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大鼠脊髓损伤BDNF基因修饰神经干细胞移植后HRP逆行示踪
引用本文:李巍,李成仁,蔡文琴,周德山.大鼠脊髓损伤BDNF基因修饰神经干细胞移植后HRP逆行示踪[J].第三军医大学学报,2003,25(1):47-50.
作者姓名:李巍  李成仁  蔡文琴  周德山
作者单位:第三军医大学基础医学部组织学与胚胎学教研室,重庆,400038
基金项目:第三军医大学校管课题 ( 1999)
摘    要:目的:检测大鼠脊髓损伤脑源性神经营养因子(Brain-derived neurotrophic factor,BDNF)基因修饰神经干细胞移植后,神经纤维的再通及后肢功能恢复情况。方法:大鼠L4脊髓全横断后,在横断处立即移植BDNF基因修饰神经干细胞,分四个时相点(1周,1、2、3个月)进行辣根过氧化物酶(HRP)逆行示踪,并观察损伤移植处的形态学变化及大鼠后肢运动功能恢复情况。结果:损伤移植处脊髓的形态学明显好转;损伤移植处上段脊髓中,移植1个月组有HRP阳性细胞,以后两组逐渐增多;移植组大鼠后肢运动功能明显恢复。结论:大鼠脊髓损伤BDNF基因修饰神经干细胞移植后,在损伤移植处有HRP阳性神经元和神经纤维,大鼠后肢运动功能明显恢复,提示BDNF基因修饰神经干细胞有修复大鼠脊髓损伤的作用。

关 键 词:神经干细胞移植  脊髓损伤  基因修饰  神经干细胞  HRP  SCI
文章编号:1000-5404(2003)01-0047-04
修稿时间:2002年9月10日

Retrograde HRP tracing after transplantation of BDNF genetically modified NSCs at spinal cord injury in rat
LI Wei,LI Cheng ren,CAI Wen qin,ZHOU De shan.Retrograde HRP tracing after transplantation of BDNF genetically modified NSCs at spinal cord injury in rat[J].Acta Academiae Medicinae Militaris Tertiae,2003,25(1):47-50.
Authors:LI Wei  LI Cheng ren  CAI Wen qin  ZHOU De shan
Abstract:Objective To investigate the nerve recanalization and the motor function of hind legs after transplantation of BDNF genetically modified neural stem cells(NSCs) at spinal cord injury site in rat. Methods After L4 spinal cord transection of rat, BDNF genetically modified NSCs were transplanted immediately. Retrograde HRP tracing through sciatic nerve were practiced at 1 week, 1 month, 2 month, 3 month after transplantation of BDNF genetically modified NSCs. The morphological changes were observed at section of spinal cord and the motor functions of both hind legs of rat were detected. Results The morphology of the injured spinal cord sections turned better. Retrograde HRP tracing through sciatic nerve showed some HRP positive neurons and nerve fibers at the site of near rostral end of the nearly injured part at one month after transplantation and increased with the time going by. Motor function of hind legs of rats recovered significantly in all transplantation groups. Conclusion BDNF genetically modified NSCs have repairing effect on spinal cord injury in rat.
Keywords:spinal cord injury  genetically modification  neural stem cell  HRP
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