湘潭地区尖锐湿疣人乳头瘤病毒感染的分型研究

目的了解湘潭地区尖锐湿疣(Condyloma Acuminatum,CA)患者感染人乳头瘤病毒(hunan papilooma virus,HPV)的基因类型及分布。方法分别用原位杂交(In situ hybridization,ISH)和免疫组化(immunohistiochemistry,IHC)方法对临床和/或病理组织学诊断CA的266例病例进行检测。结果 IHC检测HPV-P阳性166例,HPV-16阳性32例,总阳性率为65.41%(174/266),阳性细胞主要分布在棘细胞上层及颗粒细胞层;ISH检测HPV6/11阳性235例,HPV16/18阳性42例,HPV16/18阳性的42例中有24例合并有HPV6/11阳性。ISH总阳性率为95.11%(253/266),HPV6/11、HPV16/18同时感染57.14%,阳性细胞广泛出现在棘层中上层、颗粒层及角化不全细胞核内。结论本地区CA以HPV6/11感染为主,少数为高危型HPV16/18型;有较高的混合感染率。两种检测方法相比较,原位杂交技术阳性率和阳性细胞数均显著高于免疫组化技术,因此是诊断和研究CA的较好和先进的方法。

Genotype of Human Papilloma Virus among Infectious Patients with Condylima Acuminatum

【Objective】To explore the genotype and distribution of human papilloma viruses among infectious patients with condyloma acuminatum（CA） in Xiangtan region.【Methods】266 specimens diagnosed by clinincal pathol-ogy were examined with ISH and IHC.【Results】Of all specimens,166 cases were positive for HPV-P and 32 positive for HPV-16 by IHC.Overall positive rate is 65.41%（174 /266）.Expression of HPV-P and HPV-16 was evident in the upper spinous and granular layer.235 cases were positive for HPV6 /11 and 42 cases were positive for HPV16 /18,24 of which were positive for HPV6 /11 by ISH.The overall positive rate of HPV expression by ISH is 95.11% and the positive rate of double infections（HPV6 /11 ＋ HPV16 /18） analyzed by ISH was 57.14%.In addition to the upper spinal stratum,granular stratum and parakeratotic necleus.【Conclusions】The genotype of HPV6 /11 are the main type as well as little HPV16 /18 causing CA in Xiangtan region.Compared to IHC,ISH had a higher positive rate and more positive cells in continuous section.