| 甘草糖提取物对小肠上皮细胞迁移及多胺含量影响的研究 |
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| 引用本文： | 温鹏,随晶晶,李茹柳,赵世清,卢文彪,陈蔚文.甘草糖提取物对小肠上皮细胞迁移及多胺含量影响的研究[J].中药材,2012(7):1112-1116. |
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| 作者姓名： | 温鹏 随晶晶 李茹柳 赵世清 卢文彪 陈蔚文 |
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| 作者单位： | 广州中医药大学;上海市高校中医内科学E-研究院/上海中医药大学 |
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| 基金项目： | 国家自然科学基金项目(30772753,81173254);上海市教育委员会E-研究院建设计划项目(E03008) |
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| 摘 要： | 目的:观察甘草糖提取物对小肠上皮细胞(IEC-6)迁移及细胞内多胺(腐胺、精脒和精胺)含量的影响。方法:划痕法造细胞损伤后迁移模型,柱前衍生-高效液相色谱法测定细胞内多胺含量。观察甘草糖复合物对细胞迁移及细胞内多胺含量的影响;观察甘草糖复合物对二氟甲基鸟氨酸(DFMO)致细胞迁移抑制和多胺合成抑制的影响。结果:甘草糖提取物(50、100 mg/L)能促进细胞迁移;可逆转由DFMO所致的细胞迁移抑制;可提高细胞迁移过程中细胞内多胺含量;可逆转由DFMO所致的多胺含量降低。结论:甘草对胃肠黏膜损伤的修复作用可能与其提高细胞多胺含量、促进细胞迁移有关。
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| 关 键 词： | 甘草 糖复合物 多胺 小肠上皮细胞 细胞迁移 |
Effect of Polysaccharides from Radix Glycyrrhizae on Migration and Polyamines Contents of IEC-6 Cell |
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| WEN Peng,SUI Jing-jing,LI Ru-liu,ZHAO Shi-qing,LU Wen-biao,CHEN Wei-wen.Effect of Polysaccharides from Radix Glycyrrhizae on Migration and Polyamines Contents of IEC-6 Cell[J].Jorunal of Chinese Medicinal Materials,2012(7):1112-1116. |
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| Authors: | WEN Peng SUI Jing-jing LI Ru-liu ZHAO Shi-qing LU Wen-biao CHEN Wei-wen |
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| Institution: | 1,2(1.Piwei Institute,Guangzhou University of Chinese Medicine,Guangzhou 510405,China;2.E-Institute of Shanghai Municipal Education Commission,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China) |
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| Abstract: | Objective:To study the effect of polysaccharides from Radix Glycyrrhizae on migration and polyamines(putrescine,spermidine and spermine) contents of IEC-6 cell.Methods:Cell migration model was induced by scratch method in each well,and the polyamines in IEC-6 cell was determined by pre-column derivation high performance liquid chromatography.The polysaccharides inhibited effect on migration and polyamines contents of IEC-6 cells,and on IEC-6 cell migration by DFMO(a polyamines synthesis inhibitor) and the polyamines contents in the cells were observed.Results:The polysaccharides(50 mg/L or 100 mg/L) was able to promote the cell migration,reverse the cell migration inhibition by DFMO,enhance the IEC-6 cell polyamines(putrescine,spermidine and spermine) contents in the process of cell migration and reverse the reduction of polyamines(putrescine,spermidine and spermine) induced by DFMO.Conclusion:The effect of Radix Glycyrrhizae on the gastrointestinal mucosal damage repairing may be related to increasing polyamine content in cells and promoting cell migration. |
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| Keywords: | Radix Glycyrrhizae Polysaccharides Polyamine IEC-6 cell Cell migration |
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