补青颗粒防治大鼠D-乳糖性白内障的研究

目的:观察补青颗粒对大鼠半乳糖性白内障的防治作用.方法:用SD大鼠复制半乳糖白内障模型.实验分为6组:空白对照组无特殊处理；模型对照组每日ip注射50％ D-半乳糖溶液(10g·kg-1),连续15d,制成白内障动物模型；阳性对照组同模型组处理的同时每天给予障眼明片混悬液0.02g·kg-1 ig至实验结束；补青颗粒高、中、低剂量组处理同模型组且每天分别给予0.8,0.4,0.2 g·kg-1的剂量ig至实验结束.分别记录实验前及造模起第3,7,14天晶状体的混浊程度,实验结束时检测各组晶体超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量.结果:对照组晶状体始终透明,模型组第3天晶体开始发生混浊,补青颗粒3个剂量组晶体混浊的速度及程度在3,7,14天均明显低于模型组(P＜0.05),SOD活性显著高于模型组(P＜0.05),MDA含量显著低于模型组(P＜0.05).结论:补青颗粒具有较好的防治大鼠半乳糖性白内障的作用.

Experimental Study on Protection and Treatment of Galactose Cataract by Buqing Granules

Objective:To study the protection and treatment effects of Buqing granules on galactose cataract. Method: Animal model of galactose cataract was induced in SD rats.The rats were divided into six groups:black control group, model group treated by daily intraperitoneal injection of 50% D-galactose solution (10 g·kg^-1) for 15 days,positive control group treated by Zhangyan uspension 0.02 g·kg^-1 everyday,high, medium and low dose groups of Buqing granules (0.8, 0.4, 0.2 g·kg^-1). Recorded the lens turbidity was recorded before and after modeling from the first day to 3,7,14.At the end of the experiment,superoxide dismutase (SOD) and malondialdehyde (MDA) was detected. Result: The lens in control group was transparent, in model group lens opacities occurred at the third day,in the three dose groups of Buqing granules occurring time and extent of lens opacities at 3, 7 and 14 days were significantly lower than the model group(P<0.05). SOD activity was significantly higher than the model group(P<0.05),MDA was significantly lower than that in the model group (P<0.05). Conclusion: The Buqing granules had better prevention and treatment in rats with galactose cataracts.