左归丸对去卵巢大鼠股骨中核心结合因子α1 mRNA表达的影响

目的:观察左归丸对去卵巢骨质疏松大鼠股骨中核心结合因子α1 mRNA表达的影响,探讨其对骨质疏松的防治机制.方法:280只SPF级SD雌性大鼠,分为3组:正常组40只、假手术组40只、其余采取双侧背部卵巢切除术进行绝经后骨质疏松症造模.21 d后,随机分为5组:模型组、尼尔雌醇组、左归丸高、中、低剂量组.左归丸高、中、低剂量组分别给予ig6.4,3.2,1.6 g·kg-1剂量的左归丸混悬液,1次/d;尼尔雌醇组ig尼尔雌醇混悬液0.21 mg·kg-1,每周1次.于连续给药60,120,180 d,分别取大鼠左后肢股骨近端1/3部分,采用RT-PCR方法测定核心结合因子α1的mRNA表达水平.结果:与正常组比较,模型组股骨中核心结合因子α1 mRNA水平明显降低(P＜0.01)；和模型组比较,给药60,120,180 d的左归丸各剂量组,股骨中核心结合因子α1 mRNA水平显著升高(P＜0.05).结论:骨组织中核心结合因子α1 mRNA表达水平的下调可能是PMOP发生的重要机制之一,左归丸能够上调骨组织中核心结合因子α1 mRNA表达,从而有效防治骨质疏松.

Expressions of Core Binding Factor Alpha 1 mRNA of Femoral Organization by Zuogui Pill in Ovariectomy-induced Osteoporosis Rats

Objective:To observe the expression of core binding factor alpha 1 mRNA of femoral organization by Zuogui Pill in ovariectomy-induced osteoporosis rats. Method: Two hundred and eighty male SPF SD rats were divided into three groups:normal group 40;sham-operated group 40;ovariectomy-induced osteoporosis model was adopted, 21 days later, randomly modeling rats were divided into 5 groups:the model group, nilestriol group, Zuogui Pill high, medium, low dose group. Zuogui Pill groups were ig given with Zuogui Pill at dosage of 6.4, 3.2, 1.6 g·kg^-1, once per day. Nilestriol group was treated with Nilestriol liquid. The normal group, sham-operated group and the model group were given with distilled water.60, 120, 180 d later, the expression of core binding factor alpha 1 in the femoral organization was determined by RT-polymerase chain reaction (PCR). Result: Compared with the normal group, in the model group the femoral core binding factor alpha 1 mRNA level obviously reduced (P<0.01). And compared with the model group, Zuogui Pill groups increased femoral core binding factor alpha 1 mRNA level significantly(P<0.05). Conclusion: The down-regulated expression of core binding factor alpha 1 mRNA of bone tissue may be contributed to PMOP, Zuigui Pill can up-regulated mRNA expression.