TTV核酸模板快速制备方法

目的 寻求建立适合大批量临床标本检测TT病毒（TTV）核酸快速制备方法，直接用于PCR扩增。方法 应用蛋白酶K裂解酚氯仿抽取法与直接快速裂解法，分别制备TTV核酸做PCR模板，用于TTV的聚合酶链反应的快速检测。结果 在30例非甲－非庚型肝炎血清和50例慢性乙型肝炎患血清，应用经典的蛋白酶K裂解酚氯仿抽取法和直接快速裂解法，分别制备TTV核酸模板，在相同条件下进行PCR扩增，结果两种制备方法的符合率为89％。结论 TTV是一种新发现的肝炎病毒，目前主要以实验室检测病毒核酸的存在为诊断依据，建立了具有快速、简便、适合大批量临床标本中TTV核酸制备方法，对TTV肝炎的快速诊断，具有重要的意义。

Rapid preparation of transfusion transmitted virus nucleic acid template

AIM To establish a method adapting to rapid preparation of TTV DNA from bulk samples for PCR. METHODS TTV nucleic acid template was prepared for PCR using two methods, viz. hydroxybenzenechloro form extraction and directly rapid cracking. RESULTS DNA abstracted by the two methods was amplified at same PCR parameter and the coincidence rate was 89%. CONCLUSION TTV is a new discovered hepatitis virus and the main laboratory diagnosis method is to detect it's DNA by PCR, so it is very important to establish a method for rapid preparation of TTV DNA from bulk samples.