| MTA1基因表达与人骨肉瘤细胞浸润和转移的关系 |
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| 引用本文： | 李新志,陈安民,易成腊,郭风劲,罗政强,曾恒,徐卫国.MTA1基因表达与人骨肉瘤细胞浸润和转移的关系[J].中德临床肿瘤学杂志,2006,5(3):200-203. |
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| 作者姓名： | 李新志 陈安民 易成腊 郭风劲 罗政强 曾恒 徐卫国 |
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| 作者单位： | [1]Department of Orthopaedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030, China [2]Department of Traumatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030, China |
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| 摘 要： | Objective： To compare the expression level of metastasis associated-1 （MTA1） in the higher and lower metastasis sublines of human osteosarcoma cells （MG63）, and to investigate the relationship between the expression level of MTA1-EGFP and in vitro invasion and metastasis of human osteosarcoma cells. Methods： The expression level of MTA1 in two sublines of MG63 cells was detected by semi-quantitative RT-PCR, and cell invasion assay and cell proliferation assay were used to evaluate the invasive capacity in vitro in two sublines. The lower metastasis line of MG-63 cells were transfected with MTA1-EGFP full-length cDNA expression plasmid by lipofectamine. The changes of the MTA1-EGFP expression and in vitro invasion potential were measured after transfection. Results： M8 subline expressed significantly higher level of MTA1 than that of M6 subline by RT-PCR. The invasive potentials of low metastasis MG63 cell line were increased after MTA1 gene transfection. Conclusion： There may be a relationship between MTA1 and invasive potentials of human osteosarcoma cells, and MTA1 may play a role in the molecular mechanism of tumor metastases and be a potential target for gene therapy of osteosarcoma. Further studies of MTA1 in human ostersarcoma cell metastasis are needed.
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| 关 键 词： | 肿瘤转移相关基因1 骨肉瘤 浸润 转移 |
| 收稿时间： | 2005-06-29 |
| 修稿时间： | 2005-11-13 |
Relationship between the expression of MTA1 gene and invasion and metastasis of human osteosarcoma cells |
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| Xinzhi Li,Anmin Chen,Chengla Yi,Fengjin Guo,Zhenqiang Luo,Heng Zeng,Weiguo Xu.Relationship between the expression of MTA1 gene and invasion and metastasis of human osteosarcoma cells[J].The Chinese-German Journal of Clinical Oncology,2006,5(3):200-203. |
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| Authors: | Xinzhi Li Anmin Chen Chengla Yi Fengjin Guo Zhenqiang Luo Heng Zeng Weiguo Xu |
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| Institution: | (1) Department of Orthopaedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China;(2) Department of Traumatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China |
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| Abstract: | Objective: To compare the expression level of metastasis associated-1 (MTA1) in the higher and lower metastasis sublines of human osteosarcoma cells (MG63), and to investigate the relationship between the expression level of MTA1-EGFP and in vitro invasion and metastasis of human osteosarcoma cells. Methods: The expression level of MTA1 in two sublines of MG63 cells was detected by semi-quantitative RT-PCR, and cell invasion assay and cell proliferation assay were used to evaluate the invasive capacity in vitro in two sublines. The lower metastasis line of MG-63 cells were transfected with MTA1-EGFP full-length cDNA expression plasmid by lipofectamine. The changes of the MTA1-EGFP expression and in vitro invasion potential were measured after transfection. Results: M8 subline expressed significantly higher level of MTA1 than that of M6 subline by RT-PCR. The invasive potentials of low metastasis MG63 cell line were increased after MTA1 gene transfection. Conclusion: There may be a relationship between MTAl and invasive potentials of human osteosarcoma cells, and MTAl may play a role in the molecular mechanism of tumor metastases and be a potential target for gene therapy of osteosarcoma. Further studies of MTA1 in human ostersarcoma cell metastasis are needed. |
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| Keywords: | metastasis associated-1 osteosarcoma invasion neoplasm metastasis |
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