An enzyme‐linked immunosorbent assay for pyridoxamine and its comparison with alternative analytical procedures

A pyridoxal‐protein conjugate was used as an immunogen to raise antisera in rabbits. All sera recognized pyridoxamine strongly and specifically, except one which also recognized pyridoxol. An enzyme‐linked immunosorbent assay (ELISA) for pyridoxamine was set up using the microtitation plate format. Comparison of results obtained with the ELISA on 11 different food samples (ELISA limit of detection 7 pg per well) with those obtained with high performance liquid chromatography (HPLC) showed good agreement except for beef and chicken samples. Comparison of HPLC results with those obtained by microbiological procedures also showed good agreement except for potato and cabbage samples. Food table data for samples at higher levels of B6 did not show good agreement with data derived by HPLC or microbiological procedures.