8—甲氧基补骨脂素对粘液表皮样癌细胞的抑制作用

目的：研究８－甲氧基补骨脂素（８－ＭＯＰ）对粘液表皮样癌细胞（ＭＥＣ－１）的抑制作用。方法：应用常规ＭＴＴ法、细胞计数法和流式细胞术研究８－ＭＯＰ作用于粘液表皮样癌细胞的剂量－效应关系和时间－效应关系以及３０％抑制浓度的药物对细胞群体倍增时间和细胞周期的影响。结果：１）小剂量８－ＭＯＰ在短时间内对ＭＥＣ－１细胞没有抑制作用，当剂量大于２５ｍｇ／Ｌ时，药物的抑制作用呈剂量依赖性；作用时间在２４ｈ～７２ｈ之间时表现出时间依赖性关系；药物的抑制作用有延迟性。２）１２０ｈ作用组３０％抑制浓度ＩＣ３０＝３３．８６ｍｇ／Ｌ，５０％抑制浓度ＩＣ５０＝４５．３ｍｇ／Ｌ，ＲＡＡ值＝２．９。３）它阻止细胞进入Ｓ期完成细胞分裂。结论：ＭＥＣ－１细胞对８－ＭＯＰ较敏感，８－ＭＯＰ在人粘液表皮样癌的防治方面可能有一定意义。

Inhibitory effects of 8-methoxypsoralen on mucoepidermoid carcinoma MEC-1 cells

Objective:To study the inhibitory effects of 8-MOP on mucoepidermoid carcinoma MEC-1 cells. Methods:MEC-1 cells were exposed to 8-MOP at various concentrations for various times,the inhibitory effects were measured by MTT assay. Growth curve and the doubling time (DT) of the cells were obtained by cell counting. Cell phase distribution was measured with flow cytometry after the treatment of the cells with 8-MOP at IC 30 . Results:1)8-MOP inhibited the cell growth in a dose-dependent manner at concentrations between 25 mg/L and 100 mg/L, and in a time-dependent manner with 24 h to 72 h exposure. 2)The IC 50 , IC 30 and RAA values of 8-MOP against MEC-1 cells were 45.3 mg/L, 33.86 mg/L and 2.9 respectively. 3)The inhibitory effects lasted for some time after 8-MOP treatment. It arrested MEC-1 cells at G 1 phase. Conclusion:8-MOP may inhibit MEC-1 cells growth and may be potential in treating mucoepidermoid carcinoma.