Screening of Antifungal Azole Drugs and Agrochemicals with an Adapted alamarBlue-Based Assay Demonstrates Antibacterial Activity of Croconazole against Mycobacterium ulcerans |
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| Authors: | Nicole Scherr Katharina R?ltgen Matthias Witschel Gerd Pluschke |
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| Affiliation: | aSwiss Tropical and Public Health Institute, Basel, Switzerland;bUniversity of Basel, Basel, Switzerland;cBASF SE, Ludwigshafen, Germany |
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| Abstract: | An alamarBlue-based growth inhibition assay has been adapted for the thermosensitive and slow-growing pathogen Mycobacterium ulcerans. The standardized test procedure enables medium-throughput screening of preselected compound libraries. Testing of a set of 48 azoles with known antifungal activity led to the identification of an imidazole antifungal displaying an inhibitory dose (ID) of 9 μM for M. ulcerans. |
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