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C-27 and C-3 Glucosylation of Diosgenin by Cell Suspension Cultures of Costus Speciosus
Authors:Gunawan Indrayanto   Siti Zumaroh  Achmad Syahrani  Alistair L. Wilkins
Affiliation: a Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmacy, Airlangga University, Surabaya, Indonesiab Department of Chemistry, School of Science and Technology, The University of Waikato, Hamilton, New Zealand
Abstract:3-O-[β-D-glucopyranosyl-(1' → 2')-β-D-glucopyranosyl], 27-O-β-D-glucopyranosyl-(25R)-spirost-5-ene-3β,27-diol was isolated from cell suspension cultures of Costus speciosus, following incubation with diosgenin, and its structure was elucidated using a combination of one- and two-dimensional 1H and 13C NMR spectral data, and positive and negative ion ESMS spectral data.
Keywords:Costus speciosus  Cell suspension cultures  Biotransformation  Glucosylation  Diosgenin  3-O-[β-D-glucopyranosyl-(1' → 2')-β-D-glucopyranosyl]  27-O-β-D-glucopyranosyl-(25R)-spirost-5-ene-3β  27-diol
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