针刺上调脂联素信号对高脂饮食大鼠主动脉的影响

目的研究高脂饮食对sD大鼠主动脉脂联素信号的影响以及针刺能否对该影响发挥调节作用，探讨针刺的作用机制。方法高脂饲料喂养17只刚断乳的sD大鼠12周后，根据体重随机分为针刺组和模型组，干预4周后，ELISA法检测血清脂联素，实时荧光RT-PCR和western blotting分别检测主动脉脂联素受体1、2基因和蛋白，免疫组化法检测主动脉bcI-2和bax，硝酸还原酶法测主动脉一氧化碳（N0）含量，精氨酸催化法测主动脉一氧化碳合酶（NOS）活力，黄嘌呤氧化酶法测超氧化物歧化酶（SOD）活力，硫代巴比妥酸法测丙二醛（MDA）含量。并设普通饲料对照组。结果模型组血清adiponectin、主动脉AdipoR1／2mRNA和蛋白、N0、结构NOS（cNOS）、bc1-2和SOD明显低于对照组，MDA和bax明显高于对照组。针刺组血清adiponectin、主动脉AdipoRl／2mRNA和蛋白、NO、cNOS、bc1-2和SOD明显高于模型组，MDA和bax明显低于模型组。结论高脂饮食造成了sD大鼠主动脉脂联素信号转导障碍，针刺通过增加脂联素受体2基因和蛋白的表达增加了主动脉脂联素信号的传递，保护了主动脉。

Protective Effect of Acupuncture by Upregulating Signals of Adiponectin on Aorta in High-fat Dietary Induced Rats

Objective To study the effect of high-fat dietary on aortal adiponectin signal in Sprague Dawley rats,and in which whether acupncture can regulate it.Method Seventeen 3-week Sprague Dawley rats raised with high-fat dietary for 12 weeks were divided into acupuncture group and model group according to body weight.Serum level of adiponectin was detected with ELISA,aortal mRNA expressions and protein contents of adipoR1/2 were examined with real-time PCR and western blotting respectively,and aortal intimal cont...