| 碱性成纤维细胞生长因子作用血管内皮细胞后c-myc基因mRNA表达的实验研究 |
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| 引用本文： | 周志钢,何清濂,林子豪,杨松林,朱晓海.碱性成纤维细胞生长因子作用血管内皮细胞后c-myc基因mRNA表达的实验研究[J].中华整形外科杂志,2002,18(5):299-301. |
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| 作者姓名： | 周志钢 何清濂 林子豪 杨松林 朱晓海 |
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| 作者单位： | 1. 武警上海总队医院烧伤整形科,201103
2. 200003,上海,第二军医大学长征医院整形外科 |
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| 摘 要： | 目的探讨碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)促进血管内皮细胞增殖的内在分子生物学机理.方法应用组织细胞培养及分子生物学Northern blot技术,检测经bFGF作用的血管内皮细胞中c-myc基因mRNA的表达情况.结果 bFGF作用血管内皮细胞后c-myc基因mRNA的表达增加(P＜0.05),可在2 h达到高峰,并与bFGF的剂量和作用时间有关.结论 bFGF促进血管内皮细胞增殖、分裂的作用与早期反应基因c-myc的激活与表达密切相关,c-myc基因的参与可能是血管内皮细胞由静止期进入增殖期的启动因素之一.
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| 关 键 词： | c－myc基因 实验研究 碱性成纤维细胞生长因子 血管内皮细胞 血管形成 bFGF 分子生物学 基因表达 |
| 修稿时间： | 2001年5月16日 |
An experimental study on c-myc gene mRNA expression of endothelial cell induced by bFGF |
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| ZHOU Zhigang,HE Qinglian,LIN Zihao,YANG Songlin,ZHU Xiaohai.An experimental study on c-myc gene mRNA expression of endothelial cell induced by bFGF[J].Chinese Journal of Plastic Surgery,2002,18(5):299-301. |
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| Authors: | ZHOU Zhigang HE Qinglian LIN Zihao YANG Songlin ZHU Xiaohai |
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| Institution: | Department of Plastic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. |
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| Abstract: | Objective\ To investigate the molecular biological mechanism of endothelial cell proliferation induced by bFGF. Methods\ Cultured rat myocardial microvascular endothelial cells were stimulated with bFGF of various concentrations. By northern blot analysis, the levels of c\|myc mRNA expression were detected. Results\ The expression of c\|myc mRNA in the bFGF\|treated groups increased ( P <0 05) with a dose\| and stimulating time\|dependent manner. The c\|myc mRNA expression reached to a peak level at 2 hours. Conclusions\ C\|myc expression may be an important component in controlling the transit of cells through the cell cycle. |
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| Keywords: | Basic fibroblast growth factor Gene Endothelial cell Angiogenesis |
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