培养基培养法和细胞培养法分离培养生殖支原体的初步探讨

目的用培养基培养法和细胞培养法分离培养临床标本中的生殖支原体(Mg)，以证实天津地区性传播疾病(STD)门诊患者有无Mg感染。方法用无菌棉拭子取STD门诊患者生殖泌尿道分泌物，分别接种于SP-4培养基和Vero细胞进行Mg的分离培养，对符合Mg培养特性的培养物，用套式PCR试验、代谢抑制试验以及PCR扩增产物核酸序列测定等方法进行鉴定。结果采用培养基培养法自79名STD门诊患者中分离培养出2株Mg，分离率为2.53％；采用细胞培养法自80名患者中分离培养出5株Mg，分离率为6.25％。两者无显著性差异(x2=0.57，0.25<P<0.5)。结论首次同时应用培养基培养法和细胞培养法从临床标本中分离培养出Mg茵株，从病原学上证实天津地区STD患者中存在Mg感染。

Preliminary Study on Method of Cell Culture and Medium Culture for Isolation of Mycoplasma Genitalium

Objective The purpose of the observation was to prove whether there exists an infection with Mycoplasma g~(Mg) in clinical specimens from the ant-patients by using the method of cell culture and the medium culture in Tianjin. Method The clinical specimens taken from STD clinic patients by sterilized swab were cultured in SP-4 medium and Vero cell, respectively. The clinical strains whose characteristic features met the criteria for Mg were identified with metabolize inhibition tests,PCR and so on. Result By the medium culture, there were 2 strains isolated from 79 clinical specimens with an isolation rate being 2.53% .By the cell culture,there were 5 strains isolated from 80 clinical specimens with an isolation rate being 6.25%.No significant difference was found between the results by the two methods (x2 =0.57,0.25