霍山石斛杂交种与其亲本生长生理指标及药效成分含量的比较

目的：对霍山石斛杂交种与其亲本进行生长、生理指标及主要药效成分含量的比较，为霍山石斛种质资源改良提供理论依据。方法：叶绿素含量用无水乙醇法，光合速率用CI-310便携式光合作用测定系统测定；多糖含量用浓硫酸-苯酚比色法，总生物碱含量用溴甲酚绿酸性染料比色法测定。结果：杂交种生长量积累表现为近铜皮石斛的特性，较霍山石斛有极大优势；一年生杂交种叶绿素含量及光合速率均极显著高于其亲本；杂交种二年茎与三年茎多糖含量和总生物碱含量均与霍山石斛接近。结论：杂交种在生长和药效成分积累方面结合了2个亲本之间的优势，具有很大的开发利用前景。

Comparison on growth, physiology and medicinal components of Dendrobium huoshanense hybrid and its parents

Objective: To compare the hybrid between species of Dendrobium huoshanense and its parents on growing, physiologic indexes and content of medicinal components, and provide theoretical basis for species quality improvement. Method: The chlorophyll content, the photosynthesis rate, the polysaccharides content and the alkaloids content were measured by anhydrous ethanol method, CI-310 photosynthesis determination system, colorimetry of concentrated sulphuric acid-phenol and acid dyes colorimetry respectively. Result: The growth of hybrid was close to D. moniliforme, and apparently higher than D. huoshanense. The chlorophyll content and the photosynthesis rate of one-year-hybrid were markedly higher than its parents. The content of polysaccharides and alkaloids in two-year-stem and three-year-stem of hybrid were close to that of D. huoshanense. Conclusion: The hybrid integrates superiority of parents on growth and accumulation of medicinal!components opens vast vistas for development and utilization.