原位杂交法检测表皮生长因子受体基因mRNA在大肠癌和大肠息肉中的表达

目的］检测表皮生长因子受体基因ｍＲＮＡ在大肠癌和大肠息肉中的表达．［方法］应用原位杂交法．［结果］表皮生长因子受体基因ｍＲＮＡ阳性表达率在大肠癌中为６７％（２７／４０），在移行区为４０％（１６／４０），在大肠息肉中为３６％（８／２２），与正常区的５％（２／４０）相比较有非常显著性差异；但表皮生长因子受体基因ｍＲＮＡ阳性表达与大肠癌的浸润深度、分化程度和有无淋巴结转移无关．表皮生长因子受体基因ｍＲＮＡ阳性表达部位在细胞浆内．［结论］表皮生长因子受体的过度表达与大肠癌和大肠息肉的生物学行为有一定正相关

The mRNA expression of epidermal growth factor receptor gene in carcinoma and polyp of large intestine with in situ hybridization

Objective To detect the mRNA expression of epidermal growth factor receptor gene in carcinoma and polyp of large intestine.Method To use in situ hybridization.Result The mRNA expressive positive rate of epidermal growth factor receptor gene is 67%(27/40)in carcinoma of large intestine,40%(16/40)in translated region and 36%(8/22)in polyp of large intestine, and there is obvious difference,compared with the normal mucomembrane 5%(2/40).But the positive expression of epidermal growth factor receptor gene is not related to the degree of infiltration,differentiation and metastasis.The positive cells of the mRNA expression of epidermal growth factor receptor gene appears in the plasma.Conclusion The over expression of epidermal growth factor receptor is related to the biological behavior of carcinoma and polyp of large intestine.