Immunofluorescence staining of keratin filaments in cultured epithelial cells |
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Authors: | W. Michael O'Guin Alexander Schermer Tung-Tien Sun |
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Affiliation: | (1) Departments of Dermatology and Pharmacology, New York University School of Medicine, 10016 New York, New York, USA |
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Abstract: | Summary A procedure is described for the identification of cultured epithelial cells by indirect immunofluorescence staining of keratins. Additionally, some associated techniques such as double staining, keratin antigen unmasking, cytostatic drug treatment and photography are briefly outlined. |
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Keywords: | keratin cytoskeleton monoclonal antibodies cytoplasmic filaments |
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