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Immunofluorescence staining of keratin filaments in cultured epithelial cells
Authors:W. Michael O'Guin  Alexander Schermer  Tung-Tien Sun
Affiliation:(1) Departments of Dermatology and Pharmacology, New York University School of Medicine, 10016 New York, New York, USA
Abstract:Summary A procedure is described for the identification of cultured epithelial cells by indirect immunofluorescence staining of keratins. Additionally, some associated techniques such as double staining, keratin antigen unmasking, cytostatic drug treatment and photography are briefly outlined.
Keywords:keratin  cytoskeleton  monoclonal antibodies  cytoplasmic filaments
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