实验性糖尿病高脂血症动物模型的建立与研究

目的建立发病机制与人类相似的2型糖尿病高脂血症的动物模型。方法Wistar大鼠随机分为正常对照组、正常饲料加链脲佐菌素（STZ）30mg／kg组、高脂饲料加STZ 10、30、60mg／kg组，6周后，测动物血糖、血脂，并观察饮食摄水量、尿量、体重。结果高脂饲料加STZ30mg／kg组动物血糖值为（17．13＋2.31）mm01／L，胆固醇（TC）为（21．49±5．83）mmol／L，甘油三酯（TG）为（4．31±1．01）mmol／L，低密度脂蛋白（LDL—c）为（5．02±3．94）mmol／L，极低密度脂蛋白（VLDL—C）为（15．51±4．21）mmol／L，与正常对照组相比，差异有统计学意义，P〈0．05；高脂饲料加STZ30mg／kg组动物表现为饮水多、体重减轻，与正常对照组相比，差异有统计学意义，P〈0．05结论高脂饲料加STZ30mg／kg，可建立2型糖尿病高脂血症动物模型。

Establishing and assessing animal model for type 2 diabetes mellitus with hyperlipidemia

Objective To establish the animal model resembling to type 2 diabetes mellitus with hyper lipidemia. Methods Wistar rats were divided into five groups：normal group, normal diet adding STZ 30 mg/kg, high fat diet adding STZ 10, 30, 60 mg/kg. After six weeks,blood glucose （BG）, blood lipid （BL） were measured,weight, the amount of diet, water, urine were observed. Results The blood glucose of high fat diet adding STZ 30 mg/kg group was（ 17.13±2.31）mmol/L,the concentration of TC was （21.49±5.83）mmol/L,TG was （4.31± 1.01）mmol/L, LDL-C was （5.02±3.94）mmol/L,VLDL-C was（15.51±4.21）mmol/L,compared with normal group. The blood glucose,TC ,TG,LDL-C and VLDL-C of high fat diet adding STZ 30 mg/kg group obviously increased, P〈O.OS,compared with normal group,Fat diet adding STZ 30 mg/kg group showed drinking more and weight re ducing,P〈0.05. Conclusion The way of high fat diet adding STZ 30 mg/kg injection can establish the animal model of type 2 diabetes mellitus with hyperlipidemia.