巨细胞病毒感染与自然流产的关系

目的:探讨人巨细胞病毒(HCMV)感染与自然流产的关系。方法:采用荧光定量PCR(FQ-PCR)对41例自然流产妇女(病例组)的流产组织进行HCMV DNA检测,以及采用免疫组化(IHC)检测HCMV感染阳性者流产组织局部细胞因子(IL-2、IL-10、TNF-α)的变化,并与30例正常孕妇(对照组)进行比较。结果:病例组HCMV DNA的阳性检出率为41.46%,对照组的阳性检出率为13.33%,两组HCMVDNA的阳性率经统计学检验具有显著性差异(P<0.01),HCMV阳性病例组IL-10较对照组升高(P<0.05),IL-2有所降低(P<0.05),TNF-α无显著差异。结论:HCMV感染与自然流产有关,HCMV感染会引起孕妇免疫功能进一步抑制,促进了胎儿宫内感染并可能最终导致流产。

Relationship between infections of human Cytomegalovirus and spontaneous abortion

Objective:To determine the relationship between infections of human cytomegalovirus and missed abortion and to understand the change of cytokine after human cytomegalovirus infection.Methods:Tested tissues from 41cases of spontaneous abortions of unknown cause and 30 normal pregnant women were studied by using fluorescent quantitative PCR(FQ-PCR) for human cytomegalovirus DNA and immunohistochemistry for cytokine.Results:The prevalence of human cytomegalovirus DNA was 41.46%by FQ-PCR in the sera of abortion pregnant women.There were significant differences in the control group.Three were the TNF-αand IL-10 increasing and IL-2 decrease in HCMV (+) group compared with control group, there were significant differences between them.Conclusion:The results suggested that the human cytomegalovirus infection may be the important pathogen for spontaneous abortion. HCMV infection may be contribute to immune suppression of pregnant woman and result in miscarriage finally.