恒磁场对人脐静脉内皮细胞活性及超微结构的影响

目的探讨恒磁场对血管内皮细胞活性、超微结构及凋亡的影响。方法将人脐静脉内皮细胞暴露于0.05、0.11、及5 mT恒磁场中,四唑盐比色法测定细胞增殖,采用透射电镜观察内皮细胞超微结构,流式细胞仪观测内皮细胞凋亡。结果0.05 mT恒磁场促进内皮细胞的增殖活性,0.1 mT恒磁场对细胞增殖无明显影响,1 mT、5mT恒磁场抑制细胞增殖。0.05 mT0、.1 mT1、mT恒磁场不引起细胞坏死,但1 mT恒磁场可引起部分细胞变性,5mT恒磁场不但引起细胞变性,还可以导致细胞坏死。0.05 mT、0.1 mT1、mT恒磁场没有引起细胞凋亡,5 mT恒磁场导致8.4%细胞凋亡。结论恒磁场对人脐静脉内皮细胞的生物学效应与磁感应强度有关,5 mT恒磁场对人脐静脉内皮细胞具有损害作用。

Biologic Effects of Static Magnetic Field on Proliferation and Ultra-Microstructure of Human Umbilical Vascular Endothelial Cell

Aim To investigate the effects of static magnetic field on proliferation,ultra-microstructure,apoptosis of human umbilical vascular endothelial cells. Methods The effects of static magnetics on proliferation of human umbilical vascular endothelial cells was observed by MTT;transmission electric microscopy was used to evaluate the ultra-microstructure of human umbilical vascular endothelial cells;apoptosis of human umbilical vascular endothelial cells was studied by flow cytometry and transmission electric microscopy. Results 0.05 mT magnetics can accelerate the proliferation of human umbilical vascular endothelial cells;0.1 mT magnetics have no effects on the growth of human umbilical vascular endothelial cells,however,magnetics of 1 mT,5 mT attenuate growth of human umbilical vascular endothelial cells.5 mT static magnetics could induce apoptosis and necrosis of human umbilical vascular endothelial cells. Conclusion The effects of static magnetics on human umbilical vascular endothelial cells is double edge-sword,5 mT static magnetic field does harm to human umbilical vascular endothelial cells.