大蒜油对辐射致小鼠白细胞减少的预防作用

目的：研究大蒜油对辐射引起的白细胞减少的预防作用，并初步探讨其作用机制。方法：雄性BALB/c小鼠120只，按外周血白细胞数量随机分为6组：正常对照组、辐射模型组、碳酸锂阳性对照组、大蒜油低、中、高剂量组。观察小鼠接受辐射前后外周血白细胞数量的改变以及脾脏系数、脾结节计数和骨髓DNA含量的变化。结果：与辐射模型组相比，大蒜油低、中、高剂量组外周血白细胞数量分别升高了51%、59%和350%，大蒜油中、高剂量组骨髓DNA含量分别增加了56%和82%，大蒜油中、高剂量组脾脏系数分别增加了20%和39%，大蒜油低、中、高剂量组小鼠脾结节计数分别升高了41%、65%和71%(P均＜0.05)；与碳酸锂阳性对照组相比，大蒜油高剂量组白细胞数量升高了117%，脾脏系数增加了17%(P＜0.05)。结论：大蒜油对辐射引起的白细胞减少有一定的预防作用，且效果优于升白细胞药物碳酸锂；其机制可能是刺激骨髓增生造血，同时刺激脾脏和脾结节增生造血。

arlic oil can prevent the decrease of leucocyte induced by radiation in mice

To explore the effect and mechanism of garlic oil in preventing the decrease of white blood cell (WBC) induced by radiation in mice. Methods: BALB/c male mice were distributed to 6 groups according to WBC amount: normal, radiative model, positive Li2CO3, garlic oil low, middle and high dose groups. The changes of the peripheral WBC level, the spleen's coefficient, the amount of spleen nodes and the content of DNA in marrow were measured. Results: Compared with the radiative model group, the amount of peripheral WBC of garlic oil low, middle and high dose groups was increased by 51%, 59% and 350%, respectively; The content of DNA in marrow of garlic oil middle and high dose groups was enhanced by 56% and 82%, respectively; The spleen′s coefficient of garlic oil in middle and high dose groups was advanced by 20% and 39%; The amount of spleen nodes of garlic oil in low, middle and high dose groups were increased by 41%, 65% and 71% (P＜0.05). Compared with Li2CO3 positive group, the amount of peripheral WBC of garlic oil in high dose group was increased by 117%; The spleen′s coefficient of garlic oil in high dose group was advanced by 17% (P＜0.05). Conclusion: Garlic oil can prevent the WBC decrease induced by radiation at some extent possibly by stimulating the marrow hyperplasia, the spleen and the spleen nodes hyperplasia.